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Literature summary for 2.7.4.8 extracted from

  • Shimono, H.; Sugino, Y.
    Metabolism of deoxyribonucleotides. Purification and properties of deoxyguanosine monophosphokinase of calf thymus (1971), Eur. J. Biochem., 19, 256-263.
    View publication on PubMed

General Stability

General Stability Organism
dialysis against water, pH 6 with several changes of outer fluid inactivates, stable to dialysis for less than 36 h without changing the outer fluid Bos taurus
dilution inactivates calf thymus enzyme, KCl protects, not bovine serum albumin Bos taurus
KCl does not prevent heat inactivation Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
dGMP competitive inhibitor to GMP Bos taurus
GMP competitive inhibitor to dGMP Bos taurus
Li+
-
Bos taurus
Li+
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
GMP
-
Bos taurus
0.4
-
dGMP
-
Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation Rattus norvegicus
Ca2+ activation Bos taurus
Co2+
-
Rattus norvegicus
Co2+
-
Bos taurus
Fe2+ no activator Bos taurus
Fe3+ no activator Bos taurus
K+ activation Rattus norvegicus
K+ activation Bos taurus
K+ dGMP, not GMP as substrate Rattus norvegicus
K+ dGMP, not GMP as substrate Bos taurus
Mg2+ requirement Rattus norvegicus
Mg2+ requirement Bos taurus
Mn2+ requirement Rattus norvegicus
Mn2+ requirement Bos taurus
additional information
-
Rattus norvegicus
additional information GMP-kinase activity is less sensitive to metal ions than dGMP-kinase activity Bos taurus
Na+ activation Bos taurus
NH4+ activation Rattus norvegicus
NH4+ activation Bos taurus
NH4+ less effective than K+ Rattus norvegicus
NH4+ less effective than K+ Bos taurus
Ni2+ activation Bos taurus
Rb+
-
Rattus norvegicus
Rb+
-
Bos taurus
Zn2+
-
Rattus norvegicus
Zn2+
-
Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
calf
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
method that includes DEAE-cellulose, hydroxyapatite and Sephadex G-100 chromatography Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
bone marrow
-
Rattus norvegicus
-
brain
-
Rattus norvegicus
-
brain
-
Bos taurus
-
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
liver
-
Bos taurus
-
thymus
-
Rattus norvegicus
-
thymus
-
Bos taurus
-

Storage Stability

Storage Stability Organism
4°C, calf thymus enzyme, stable in the absence of thiols Bos taurus
4°C, diluted calf thymus enzyme solution, 0.002 mg/ml, inactivation within 10 days, KCl protects Bos taurus
4°C, partially purified calf thymus enzyme preparation in the presence of 1 M KCl, 3 months Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + dGMP
-
Rattus norvegicus ADP + dGDP
-
r
ATP + dGMP
-
Bos taurus ADP + dGDP
-
r
ATP + GMP specificity Rattus norvegicus ADP + GDP
-
?
ATP + GMP specificity Bos taurus ADP + GDP
-
?
ATP + GMP specificity Bos taurus ADP + GDP
-
r
ATP + GMP no donor substrates are ITP, dGTP, dCTP or dTTP Bos taurus ADP + GDP
-
?
ATP + GMP no donor substrates are ITP, dGTP, dCTP or dTTP Bos taurus ADP + GDP
-
r
ATP + GMP dCMP is no acceptor substrate Rattus norvegicus ADP + GDP
-
?
ATP + GMP dCMP is no acceptor substrate Bos taurus ADP + GDP
-
?
ATP + GMP dCMP is no acceptor substrate Bos taurus ADP + GDP
-
r
ATP + GMP dTMP is no acceptor substrate Bos taurus ADP + GDP
-
?
ATP + GMP dTMP is no acceptor substrate Bos taurus ADP + GDP
-
r
ATP + GMP AMP is no acceptor substrate Rattus norvegicus ADP + GDP
-
?
ATP + GMP AMP is no acceptor substrate Bos taurus ADP + GDP
-
?
ATP + GMP AMP is no acceptor substrate Bos taurus ADP + GDP
-
r
ATP + GMP dAMP is no acceptor substrate Rattus norvegicus ADP + GDP
-
?
ATP + GMP dAMP is no acceptor substrate Bos taurus ADP + GDP
-
?
ATP + GMP dAMP is no acceptor substrate Bos taurus ADP + GDP
-
r
ATP + GMP no donor substrates are GTP, CTP, UTP Bos taurus ADP + GDP
-
?
ATP + GMP no donor substrates are GTP, CTP, UTP Bos taurus ADP + GDP
-
r
ATP + GMP CMP, UMP are no acceptor substrates Rattus norvegicus ADP + GDP
-
?
ATP + GMP CMP, UMP are no acceptor substrates Bos taurus ADP + GDP
-
?
ATP + GMP CMP, UMP are no acceptor substrates Bos taurus ADP + GDP
-
r
dATP + dGMP
-
Rattus norvegicus dADP + dGDP
-
?
dATP + dGMP as good as ATP Bos taurus dADP + dGDP
-
?
dATP + GMP
-
Rattus norvegicus dADP + GDP
-
?
dATP + GMP as good as ATP Bos taurus dADP + GDP
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
10 min, about 80% loss of activity, t1/2: 3 min (dGMP-phosphorylation), t1/2: 4 min (GMP-phosphorylation) Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 10 dGMP-phosphorylation Bos taurus
7 10.9 GMP-phosphorylation Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
5 10.9 about half-maximal activity at pH 5 and maximal activity at pH 10.9, GMP-phosphorylation, calf thymus Bos taurus
5.7 10.9 about half-maximal activity at pH 5.7 and 10.9, dGMP-phosphorylation, calf thymus Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.064
-
GMP pH 7.5 Bos taurus
0.57
-
dGMP pH 7.5 Bos taurus