General Stability | Organism |
---|---|
dialysis against water, pH 6 with several changes of outer fluid inactivates, stable to dialysis for less than 36 h without changing the outer fluid | Bos taurus |
dilution inactivates calf thymus enzyme, KCl protects, not bovine serum albumin | Bos taurus |
KCl does not prevent heat inactivation | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dGMP | competitive inhibitor to GMP | Bos taurus | |
GMP | competitive inhibitor to dGMP | Bos taurus | |
Li+ | - |
Bos taurus | |
Li+ | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
GMP | - |
Bos taurus | |
0.4 | - |
dGMP | - |
Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation | Rattus norvegicus | |
Ca2+ | activation | Bos taurus | |
Co2+ | - |
Rattus norvegicus | |
Co2+ | - |
Bos taurus | |
Fe2+ | no activator | Bos taurus | |
Fe3+ | no activator | Bos taurus | |
K+ | activation | Rattus norvegicus | |
K+ | activation | Bos taurus | |
K+ | dGMP, not GMP as substrate | Rattus norvegicus | |
K+ | dGMP, not GMP as substrate | Bos taurus | |
Mg2+ | requirement | Rattus norvegicus | |
Mg2+ | requirement | Bos taurus | |
Mn2+ | requirement | Rattus norvegicus | |
Mn2+ | requirement | Bos taurus | |
additional information | - |
Rattus norvegicus | |
additional information | GMP-kinase activity is less sensitive to metal ions than dGMP-kinase activity | Bos taurus | |
Na+ | activation | Bos taurus | |
NH4+ | activation | Rattus norvegicus | |
NH4+ | activation | Bos taurus | |
NH4+ | less effective than K+ | Rattus norvegicus | |
NH4+ | less effective than K+ | Bos taurus | |
Ni2+ | activation | Bos taurus | |
Rb+ | - |
Rattus norvegicus | |
Rb+ | - |
Bos taurus | |
Zn2+ | - |
Rattus norvegicus | |
Zn2+ | - |
Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
calf | - |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
method that includes DEAE-cellulose, hydroxyapatite and Sephadex G-100 chromatography | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bone marrow | - |
Rattus norvegicus | - |
brain | - |
Rattus norvegicus | - |
brain | - |
Bos taurus | - |
kidney | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
liver | - |
Bos taurus | - |
thymus | - |
Rattus norvegicus | - |
thymus | - |
Bos taurus | - |
Storage Stability | Organism |
---|---|
4°C, calf thymus enzyme, stable in the absence of thiols | Bos taurus |
4°C, diluted calf thymus enzyme solution, 0.002 mg/ml, inactivation within 10 days, KCl protects | Bos taurus |
4°C, partially purified calf thymus enzyme preparation in the presence of 1 M KCl, 3 months | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dGMP | - |
Rattus norvegicus | ADP + dGDP | - |
r | |
ATP + dGMP | - |
Bos taurus | ADP + dGDP | - |
r | |
ATP + GMP | specificity | Rattus norvegicus | ADP + GDP | - |
? | |
ATP + GMP | specificity | Bos taurus | ADP + GDP | - |
? | |
ATP + GMP | specificity | Bos taurus | ADP + GDP | - |
r | |
ATP + GMP | no donor substrates are ITP, dGTP, dCTP or dTTP | Bos taurus | ADP + GDP | - |
? | |
ATP + GMP | no donor substrates are ITP, dGTP, dCTP or dTTP | Bos taurus | ADP + GDP | - |
r | |
ATP + GMP | dCMP is no acceptor substrate | Rattus norvegicus | ADP + GDP | - |
? | |
ATP + GMP | dCMP is no acceptor substrate | Bos taurus | ADP + GDP | - |
? | |
ATP + GMP | dCMP is no acceptor substrate | Bos taurus | ADP + GDP | - |
r | |
ATP + GMP | dTMP is no acceptor substrate | Bos taurus | ADP + GDP | - |
? | |
ATP + GMP | dTMP is no acceptor substrate | Bos taurus | ADP + GDP | - |
r | |
ATP + GMP | AMP is no acceptor substrate | Rattus norvegicus | ADP + GDP | - |
? | |
ATP + GMP | AMP is no acceptor substrate | Bos taurus | ADP + GDP | - |
? | |
ATP + GMP | AMP is no acceptor substrate | Bos taurus | ADP + GDP | - |
r | |
ATP + GMP | dAMP is no acceptor substrate | Rattus norvegicus | ADP + GDP | - |
? | |
ATP + GMP | dAMP is no acceptor substrate | Bos taurus | ADP + GDP | - |
? | |
ATP + GMP | dAMP is no acceptor substrate | Bos taurus | ADP + GDP | - |
r | |
ATP + GMP | no donor substrates are GTP, CTP, UTP | Bos taurus | ADP + GDP | - |
? | |
ATP + GMP | no donor substrates are GTP, CTP, UTP | Bos taurus | ADP + GDP | - |
r | |
ATP + GMP | CMP, UMP are no acceptor substrates | Rattus norvegicus | ADP + GDP | - |
? | |
ATP + GMP | CMP, UMP are no acceptor substrates | Bos taurus | ADP + GDP | - |
? | |
ATP + GMP | CMP, UMP are no acceptor substrates | Bos taurus | ADP + GDP | - |
r | |
dATP + dGMP | - |
Rattus norvegicus | dADP + dGDP | - |
? | |
dATP + dGMP | as good as ATP | Bos taurus | dADP + dGDP | - |
? | |
dATP + GMP | - |
Rattus norvegicus | dADP + GDP | - |
? | |
dATP + GMP | as good as ATP | Bos taurus | dADP + GDP | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
10 min, about 80% loss of activity, t1/2: 3 min (dGMP-phosphorylation), t1/2: 4 min (GMP-phosphorylation) | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | dGMP-phosphorylation | Bos taurus |
7 | 10.9 | GMP-phosphorylation | Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10.9 | about half-maximal activity at pH 5 and maximal activity at pH 10.9, GMP-phosphorylation, calf thymus | Bos taurus |
5.7 | 10.9 | about half-maximal activity at pH 5.7 and 10.9, dGMP-phosphorylation, calf thymus | Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.064 | - |
GMP | pH 7.5 | Bos taurus | |
0.57 | - |
dGMP | pH 7.5 | Bos taurus |