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Literature summary for 2.7.4.8 extracted from
- The partial purification of deoxynucleoside monophosphate kinases from L cells (1965), Biochim. Biophys. Acta, 108, 114-124.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activation by EDTA | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Mus musculus |  |
| EDTA | - |
Mus musculus | |
| p-hydroxymercuribenzoate | no effect at 0.25 mM, 30% activity at 2.5 mM | Mus musculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.072 | - |
dGMP | pH 8.0, 37ºC | Mus musculus | |
| 0.26 | - |
GMP | pH 8.0, 37ºC | Mus musculus | |
| 1 | - |
ATP | pH 8.0, 37ºC, dGMP kinase activity | Mus musculus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10% as effective as Mg2+ | Mus musculus | |
| Co2+ | less than 7% as effective as Mg2+ | Mus musculus | |
| Fe2+ | activation, 90% as effective as Mg2+ | Mus musculus | |
| Fe3+ | activation, less than 7% as effective as Mg2+ | Mus musculus | |
| K+ | activation | Mus musculus | |
| K+ | activity of dGMP kinase progressively enhanced as the concentration of KCl is increased to 250 mM, GMP phosphorylation unaffected | Mus musculus | |
| K+ | dGMP, not GMP as substrate | Mus musculus | |
| Mg2+ | requirement | Mus musculus | |
| Mn2+ | requirement | Mus musculus | |
| Mn2+ | equally effective as Mg2+ | Mus musculus | |
| Zn2+ | less than 7% as effective as Mg2+ | Mus musculus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
mouse | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial by a method that includes DEAE-cellulose chromatography | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fibroblast | strain L60TM, a subline of Earle's L-strain, i.e. L-cells | Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.24 | - |
- |
Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + dGMP | - |
Mus musculus | ADP + dGDP | - |
r | |
| ATP + GMP | specificity | Mus musculus | ADP + GDP | - |
? | |
| ATP + GMP | AMP is no acceptor substrate | Mus musculus | ADP + GDP | - |
? | |
| ATP + GMP | dAMP is no acceptor substrate | Mus musculus | ADP + GDP | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
incubation for 2 min, 5 min, 10 min or 20 min leads to 43%, 67%, 89% or 97% loss of activity, respectively, 30 min: inactivation | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | equal activity in Tris-HCl buffer and 3,3-dimethylglutarate buffer | Mus musculus |
| 8 | - |
assay at | Mus musculus |
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