Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.4.7 extracted from

  • Reddick, J.J.; Kinsland, C.; Nicewonger, R.; Christian, T.; Downs, D.M.; Winkler, M.E.; Begley, T.P.
    Overexpression, purification and characterization of two pyrimidine kinases involved in the biosynthesis of thiamin: 4-amino-5-hydroxymethyl-2-methylpyrimidine kinase and 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate kinase (1998), Tetrahedron, 54, 15983-15991.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of ThiD in Escherichia coli Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
2-methyl-4-amino-5-hydroxymethylpyrimidine phosphate pH 8.0, 25°C, recombinant ThiD Escherichia coli
0.15
-
2-methyl-4-amino-5-hydroxymethylpyrimidine pH 8.0, 25°C, recombinant ThiD Escherichia coli
0.193
-
ATP pH 8.0, 25°C, recombinant ThiD Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31232
-
4 * 31232, deduced from nucleotide sequence Escherichia coli
33000
-
4 * 33000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine Escherichia coli involved in thiamine biosynthesis ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
?
ATP + 2-methyl-4-amino-5-phosphomethylpyrimidine Escherichia coli
-
ADP + 2-methyl-4-amino-5-diphosphomethylpyrimidine
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
his-tagged recombinant ThiD Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine
-
Escherichia coli ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
?
ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine involved in thiamine biosynthesis Escherichia coli ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
?
ATP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
Escherichia coli ADP + 2-methyl-4-amino-5-diphosphomethylpyrimidine
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 33000, SDS-PAGE Escherichia coli
tetramer 4 * 31232, deduced from nucleotide sequence Escherichia coli

Synonyms

Synonyms Comment Organism
HMP-P kinase
-
Escherichia coli
ThiD
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.07
-
ATP pH 8.0, 25°C, recombinant ThiD Escherichia coli
0.07
-
2-methyl-4-amino-5-hydroxymethylpyrimidine phosphate pH 8.0, 25°C, recombinant ThiD Escherichia coli
0.0867
-
2-methyl-4-amino-5-hydroxymethylpyrimidine pH 8.0, 25°C, recombinant ThiD Escherichia coli