Cloned (Comment) | Organism |
---|---|
overexpression of ThiD in Escherichia coli | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
2-methyl-4-amino-5-hydroxymethylpyrimidine phosphate | pH 8.0, 25°C, recombinant ThiD | Escherichia coli | |
0.15 | - |
2-methyl-4-amino-5-hydroxymethylpyrimidine | pH 8.0, 25°C, recombinant ThiD | Escherichia coli | |
0.193 | - |
ATP | pH 8.0, 25°C, recombinant ThiD | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31232 | - |
4 * 31232, deduced from nucleotide sequence | Escherichia coli |
33000 | - |
4 * 33000, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine | Escherichia coli | involved in thiamine biosynthesis | ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine | - |
? | |
ATP + 2-methyl-4-amino-5-phosphomethylpyrimidine | Escherichia coli | - |
ADP + 2-methyl-4-amino-5-diphosphomethylpyrimidine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
his-tagged recombinant ThiD | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine | - |
Escherichia coli | ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine | - |
? | |
ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine | involved in thiamine biosynthesis | Escherichia coli | ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine | - |
? | |
ATP + 2-methyl-4-amino-5-phosphomethylpyrimidine | - |
Escherichia coli | ADP + 2-methyl-4-amino-5-diphosphomethylpyrimidine | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 33000, SDS-PAGE | Escherichia coli |
tetramer | 4 * 31232, deduced from nucleotide sequence | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
HMP-P kinase | - |
Escherichia coli |
ThiD | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
ATP | pH 8.0, 25°C, recombinant ThiD | Escherichia coli | |
0.07 | - |
2-methyl-4-amino-5-hydroxymethylpyrimidine phosphate | pH 8.0, 25°C, recombinant ThiD | Escherichia coli | |
0.0867 | - |
2-methyl-4-amino-5-hydroxymethylpyrimidine | pH 8.0, 25°C, recombinant ThiD | Escherichia coli |