Cloned (Comment) | Organism |
---|---|
gene TcCLB.508707.200, recombinant overexpression of GFP-tagged wild-type and mutant enzymes in transgenic epimastigotes, expression analysis, enzyme fused with dimeric GFP relocalizes into protein granules inside the cell, positioned in most cases adjacent to the nucleus, while control parasites transfected with GFP conserved the cytosolic localization, overview. The observed granules all along the parasites population are of different size probably due to the heterogeneity in the expression levels of the fusion protein. The majority of this granules occupy 5-15% of the cell body | Trypanosoma cruzi |
Protein Variants | Comment | Organism |
---|---|---|
H117N | site-directed mutagenesis, the mutant His117N fused with GFP is inactive | Trypanosoma cruzi |
P95S | site-directed mutagenesis, mutation in Pro95 and in the C-terminus destabilize the hexamer fused with GFP | Trypanosoma cruzi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Trypanosoma cruzi | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | Q4E256 | gene TcCLB.508707.200 | - |
Trypanosoma cruzi CL Brener | Q4E256 | gene TcCLB.508707.200 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epimastigote | - |
Trypanosoma cruzi | - |
trypomastigote | - |
Trypanosoma cruzi | - |
Subunits | Comment | Organism |
---|---|---|
More | the cytosolic enzyme, fused to GFP, generates large granules that depend on its quaternary, oligomeric structure, overview | Trypanosoma cruzi |
Synonyms | Comment | Organism |
---|---|---|
NDPK | - |
Trypanosoma cruzi |
nucleoside diphosphate kinase | - |
Trypanosoma cruzi |
TcNDPK1 | - |
Trypanosoma cruzi |
General Information | Comment | Organism |
---|---|---|
physiological function | nucleoside diphosphate kinase is a key enzyme in the control of cellular concentrations of nucleoside triphosphates, and plays important roles in many cellular processes | Trypanosoma cruzi |