Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.4.6 extracted from

  • Sikarwar, J.; Kaushik, S.; Sinha, M.; Kaur, P.; Sharma, S.; Singh, T.P.
    Cloning, expression, and purification of nucleoside diphosphate kinase from Acinetobacter baumannii (2013), Enzyme Res., 2013, 597028.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.6

Cloned(Commentary)

Cloned (Comment) Organism
gene ndk, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Acinetobacter baumannii

Inhibitors

Inhibitors Comment Organism Structure
Urea complete enzyme inactivation at 6 M, 50% inhibition at 5 M Acinetobacter baumannii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Acinetobacter baumannii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + nucleoside diphosphate Acinetobacter baumannii
-
 ADP + nucleoside triphosphate
-
 r

Organism

Organism UniProt Comment Textmining
Acinetobacter baumannii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis Acinetobacter baumannii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + nucleoside diphosphate
-
 Acinetobacter baumannii ADP + nucleoside triphosphate
-
 r
ATP + nucleoside diphosphate analysis of ATP binding by the enzyme at different concentrations and pH values, overview Acinetobacter baumannii ADP + nucleoside triphosphate
-
 r

Synonyms

Synonyms Comment Organism
NDK
-
 Acinetobacter baumannii

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Acinetobacter baumannii

General Information

General Information Comment Organism
physiological function nucleoside diphosphate kinase is involved in the catalysis of the transfer of gamma-phosphate from nucleoside triphosphate to nucleoside diphosphate and thus maintains the nucleotide pool. The dNTPs that are formed are used as precursors for DNA and RNA synthesis. NTPs, specifically GTP, are important for cellular macromolecular synthesis and signalling mechanisms. Ndk plays a pivotal role in crucial events like bacterial growth, signal transduction, and pathogenicity Acinetobacter baumannii