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Literature summary for 2.7.4.6 extracted from

  • Ishibashi, M.; Iwasa, T.; Kumeda, K.; Arakawa, T.; Tokunaga, M.
    Effects of mutations at Gly114 on the stability and refolding of Haloarchaeal nucleoside diphosphate kinase in low salt solution (2009), Int. J. Biol. Macromol., 44, 361-364.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Halobacterium salinarum

Protein Variants

Protein Variants Comment Organism
G114D inactive mutant enzyme, dissociates more than wild-type enzyme in low salt buffer Halobacterium salinarum
G114K active mutant enzyme, refolds in 1M NaCl after heat-denaturation, under which the wild-type enzyme and mutant enzyme G114S proteins show no refolding Halobacterium salinarum
G114R once folded, the mutant enzyme is stable even in low salt buffer Halobacterium salinarum
G114S active mutant enzyme, dissociates as extensively as the wild-type enzyme as low salt buffer Halobacterium salinarum

General Stability

General Stability Organism
mutants with a basic amino acid at residue 114 (G114K, G114R) are resistant against heat inactivation as well as against low salt dissociation Halobacterium salinarum

Organism

Organism UniProt Comment Textmining
Halobacterium salinarum P61136
-
-
Halobacterium salinarum JCM 11081 P61136
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Halobacterium salinarum

Subunits

Subunits Comment Organism
dimer G114S mutant behaves similarly to wild-type enzyme, dissociating a dimer Halobacterium salinarum
hexamer G114K mutant has a hexameric structure under low salt concentration of non-denaturing PAGE condition Halobacterium salinarum
More a basic amino acid at residue 114 is responsible for the stabilization of subunit assembly Halobacterium salinarum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
mutants with a basic amino acid at residue 114 (G114K, G114R) are resistant against heat inactivation as well as against low salt dissociation Halobacterium salinarum
30
-
G114S mutant is stable up to 30°C Halobacterium salinarum