Literature summary for 2.7.4.6 extracted from

Ishibashi, M.; Hayashi, T.; Yoshida, C.; Tokunaga, M.
Increase of salt dependence of halophilic nucleoside diphosphate kinase caused by a single amino acid substitution (2013), Extremophiles, 17, 585-591.

Protein Variants

Protein Variants	Comment	Organism
N111L	a HisN111L mutant with an N-terminal extension sequence containing hexa-His tag has enhanced salt requirements for enzymatic activity and refolding even though the secondary structure of the HisN111L mutant is confirmed to be similar to the control	Halobacterium salinarum

General Stability

General Stability	Organism
the wild-type enzyme requires NaCl at higher than 2 M NaCl for refolding. Increasing the salt concentration gradually facilitates the recovery of enzyme activity, and the recovery at 3 M NaCl is maximal. The recovery at 4 M NaCl decreases slightly. No refolding of the HisN111L mutant is detected below 1 M NaCl and slow refolding is observed at 2 M NaCl, significant recovery occurs at higher than 3 M NaCl. The refolding efficiency at 4 M NaCl is as same as that of 3 M NaCl, differently from the wild-type enzyme	Halobacterium salinarum

Organism

the wild-type enzyme requires NaCl at higher than 2 M NaCl for refolding. Increasing the salt concentration gradually facilitates the recovery of enzyme activity, and the recovery at 3 M NaCl is maximal. The recovery at 4 M NaCl decreases slightly. No refolding of the HisN111L mutant is detected below 1 M NaCl and slow refolding is observed at 2 M NaCl, significant recovery occurs at higher than 3 M NaCl. The refolding efficiency at 4 M NaCl is as same as that of 3 M NaCl, differently from the wild-type enzyme

Halobacterium salinarum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	the enzyme does not require NaCl for stability or enzymatic activity below 30°C	Halobacterium salinarum

Organism

Organism	UniProt	Comment	Textmining
Halobacterium salinarum	P61136	-	-
Halobacterium salinarum JCM 11081	P61136	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Halobacterium salinarum

Subunits

Subunits	Comment	Organism
homohexamer	assembled as a trimer of basic dimeric units	Halobacterium salinarum

Synonyms

Synonyms	Comment	Organism
nucleoside diphosphate kinase	-	Halobacterium salinarum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
45	53	the melting of the HisN111L mutant shows a transition starting at 45.2°C and ending at 52.6°C in 0.2 M NaCl	Halobacterium salinarum
47	52	the melting of the wild-type enzyme shows a transition starting at 46.7°C and ending at 51.9°C in 0.2 M NaCl	Halobacterium salinarum