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Literature summary for 2.7.4.6 extracted from
- Histidine 117 in the His-Gly-Ser-Asp motif is required for the biochemical activities of nucleoside diphosphate kinase of Mycobacterium smegmatis (2012), Open Biochem. J., 6, 71-77.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli M15 cells | Mycolicibacterium smegmatis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H117Q | the mutation abolishes the ATPase and GTPase activities of the recombinant enzyme in vitro to almost 4.75- and 2fold, respectively. The mutation also reduces the autophosphorylation activity, almost 5fold | Mycolicibacterium smegmatis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + NDP | Mycolicibacterium smegmatis | - |
ADP + NTP | - |
r |  |
| ATP + NDP | Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | - |
ADP + NTP | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycolicibacterium smegmatis | - |
- |
- |
| Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | autophosphorylation | Mycolicibacterium smegmatis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-NTA agarose affinity column chromatography | Mycolicibacterium smegmatis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + CDP | - |
Mycolicibacterium smegmatis | ADP + CTP | - |
r | |
| ATP + CDP | - |
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | ADP + CTP | - |
r | |
| ATP + GDP | - |
Mycolicibacterium smegmatis | ADP + GTP | - |
r | |
| ATP + GDP | - |
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | ADP + GTP | - |
r | |
| ATP + NDP | - |
Mycolicibacterium smegmatis | ADP + NTP | - |
r | |
| ATP + NDP | - |
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | ADP + NTP | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NDK | - |
Mycolicibacterium smegmatis |
| nucleoside diphosphate kinase | - |
Mycolicibacterium smegmatis |
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Release 2023.1 (January 2023)
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