Literature summary for 2.7.4.6 extracted from

Ishibashi, M.; Ida, K.; Tatsuda, S.; Arakawa, T.; Tokunaga, M.
Interaction of hexa-His tag with acidic amino acids results in facilitated refolding of halophilic nucleoside diphosphate kinase (2011), Int. J. Biol. Macromol., 49, 778-783.

Search for more literature for 2.7.4.6

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 Star (DE3) cells	Halobacterium salinarum

Protein Variants

Protein Variants	Comment	Organism
D112S	the mutant shows reduced specific activity compared to the wild type enzyme	Halobacterium salinarum
D58G	the mutant shows strongly reduced specific activity compared to the wild type enzyme	Halobacterium salinarum
D58G/D63G	the mutant shows no refolding enhancement and strongly reduced specific activity compared to the wild type enzyme	Halobacterium salinarum
D63G	the mutant shows slightly reduced specific activity compared to the wild type enzyme	Halobacterium salinarum
G114D	the mutant, which forms a dimer in low salt solution, is strongly stabilized by His-tag to form a stable hexamer and shows strongly reduced specific activity compared to the wild type enzyme	Halobacterium salinarum

Organism

Organism	UniProt	Comment	Textmining
Halobacterium salinarum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ATP-agarose column chromatography, and gel filtration	Halobacterium salinarum

Renatured (Commentary)

Renatured (Comment)	Organism
in 50 mM Tris-HCl buffer containing 3 M NaCl, at 4°C. Refolding is extremely slow, reaching the nearly full activity at 72 h	Halobacterium salinarum

Synonyms

Synonyms	Comment	Organism
NDK	-	Halobacterium salinarum
nucleoside diphosphate kinase	-	Halobacterium salinarum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
90	-	heating at 90°C for 5 min is sufficient to fully inactivate the protein	Halobacterium salinarum

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Release 2023.1 (January 2023)