Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLyS cells | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.079 | - |
ADP | isozyme NDPK2, with TTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
0.108 | - |
ADP | isozyme NDPK3, with TTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
0.125 | - |
ADP | isozyme NDPK3, with GTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
0.138 | - |
ADP | isozyme NDPK2, with GTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
0.392 | - |
ADP | isozyme NDPK1, with TTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
0.45 | - |
GTP | isozyme NDPK2, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
0.51 | - |
GTP | isozyme NDPK3, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
0.552 | - |
ADP | isozyme NDPK1, with CTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
0.578 | - |
ADP | isozyme NDPK3, with CTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
0.707 | - |
ADP | isozyme NDPK1, with GTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
0.89 | - |
TTP | isozyme NDPK2, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
1.26 | - |
ADP | isozyme NDPK2, with CTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
1.34 | - |
TTP | isozyme NDPK3, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
1.69 | - |
GTP | isozyme NDPK1, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
5.28 | - |
TTP | isozyme NDPK1, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
5.28 | - |
CTP | isozyme NDPK3, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
6.72 | - |
CTP | isozyme NDPK1, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
9.37 | - |
CTP | isozyme NDPK2, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | isozyme NDPK1 | Oryza sativa | 5829 | - |
mitochondrion | isozyme NDPK3 | Oryza sativa | 5739 | - |
plastid | isozyme NDPK2 | Oryza sativa | 9536 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17400 | - |
x * 17400, isozyme NDPK2, calculated from amino acid sequence | Oryza sativa |
17900 | - |
x * 17900, isozyme NDPK1, calculated from amino acid sequence | Oryza sativa |
18000 | - |
x * 18000, isozyme NDPK3, calculated from amino acid sequence | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
cultivar Kitaake | - |
Purification (Comment) | Organism |
---|---|
Ni-chelating Sepharose column chromatography | Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Oryza sativa | - |
leaf sheath | - |
Oryza sativa | - |
additional information | isoform NDPK1 is weakly expressed in leaf sheaths | Oryza sativa | - |
seed | immature seed | Oryza sativa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + CTP | - |
Oryza sativa | ATP + CDP | - |
? | |
ADP + GTP | - |
Oryza sativa | ATP + GDP | - |
? | |
ADP + TTP | - |
Oryza sativa | ATP + TDP | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 17400, isozyme NDPK2, calculated from amino acid sequence | Oryza sativa |
? | x * 17900, isozyme NDPK1, calculated from amino acid sequence | Oryza sativa |
? | x * 18000, isozyme NDPK3, calculated from amino acid sequence | Oryza sativa |
Synonyms | Comment | Organism |
---|---|---|
NDPK1 | isozyme | Oryza sativa |
NDPK2 | isozyme | Oryza sativa |
NDPK3 | isozyme | Oryza sativa |
nucleoside diphosphate kinase | - |
Oryza sativa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | after 15 min incubation at 40°C, isozymes NDPK1 and NDPK3 show about 40% and 80% residual activity and no activity at 60°C, respectively, while isozyme NDPK2 shows about 40% residual activity at 60°C | Oryza sativa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14.2 | - |
TTP | isozyme NDPK2, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
17.9 | - |
GTP | isozyme NDPK3, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
27.7 | - |
GTP | isozyme NDPK2, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
33.5 | - |
TTP | isozyme NDPK3, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
49.4 | - |
GTP | isozyme NDPK1, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
55 | - |
TTP | isozyme NDPK1, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
85.2 | - |
CTP | isozyme NDPK2, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
89 | - |
CTP | isozyme NDPK3, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
229 | - |
CTP | isozyme NDPK1, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
isozymes NDPK2 and NDPK3 | Oryza sativa |
8.4 | - |
isozyme NDPK1 | Oryza sativa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | isozyme NDPK3 | Oryza sativa |
6 | 9 | isozyme NDPK2 | Oryza sativa |
7 | 9 | isozyme NDPK1 | Oryza sativa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.1 | - |
CTP | isozyme NDPK2, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
10.4 | - |
TTP | isozyme NDPK1, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
16 | - |
TTP | isozyme NDPK2, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
16.9 | - |
CTP | isozyme NDPK3, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
25 | - |
TTP | isozyme NDPK3, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
29.2 | - |
GTP | isozyme NDPK1, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
34.1 | - |
CTP | isozyme NDPK1, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
35.1 | - |
GTP | isozyme NDPK3, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
61.6 | - |
GTP | isozyme NDPK2, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
67.6 | - |
ADP | isozyme NDPK2, with CTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
69.9 | - |
ADP | isozyme NDPK1, with GTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
140 | - |
ADP | isozyme NDPK1, with TTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
143 | - |
ADP | isozyme NDPK3, with GTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
154 | - |
ADP | isozyme NDPK3, with CTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
180 | - |
ADP | isozyme NDPK2, with TTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
210 | - |
ADP | isozyme NDPK2, with GTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
310 | - |
ADP | isozyme NDPK3, with TTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa | |
415 | - |
ADP | isozyme NDPK1, with CTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C | Oryza sativa |