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Literature summary for 2.7.4.6 extracted from
- Trypanosoma cruzi nucleoside diphosphate kinase 1 (TcNDPK1) has a broad nuclease activity (2008), Parasitology, 135, 1661-1666.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Trypanosoma cruzi |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + NDP | Trypanosoma cruzi | - |
ADP + NTP | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma cruzi | Q4E256 | - |
- |
| Trypanosoma cruzi | Q4E4R9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-agarose resin column chromatography, gel filtration | Trypanosoma cruzi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + NDP | - |
Trypanosoma cruzi | ADP + NTP | - |
? | |
| additional information | NDPK1 exhibits nuclease activity cleaving not only linear DNA, but also supercoiled plasmid DNA, ATP or ADP do not affect the nuclease activity while the absence of Mg2+ completely inhibits this activity | Trypanosoma cruzi | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NDPK1 | - |
Trypanosoma cruzi |
| NDPK2 | - |
Trypanosoma cruzi |
| nucleoside diphosphate kinase 1 | - |
Trypanosoma cruzi |
| nucleoside diphosphate kinase 2 | - |
Trypanosoma cruzi |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Trypanosoma cruzi | |
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Release 2023.1 (January 2023)
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