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Literature summary for 2.7.4.6 extracted from

  • Shen, Y.; Han, Y.J.; Kim, J.I.; Song, P.S.
    Arabidopsis nucleoside diphosphate kinase-2 as a plant GTPase activating protein (2008), BMB Rep., 41, 645-650.
    View publication on PubMed
Search for more literature for 2.7.4.6

Cloned(Commentary)

Cloned (Comment) Organism
expression of GST-tagged NPDK2 Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
H197C NDPK2 kinase site mutant Arabidopsis thaliana
H197C no enzymatic activity, stimulates GTPase activites of Pra2 and Pra3, EC 3.6.5.2, in the same manner as wild-type NDPK2 Arabidopsis thaliana
L225stop C-terminal deletion mutant Arabidopsis thaliana
L225stop increased enzymatic activity, stimulates GTPase activites of Pra2 and Pra3, EC 3.6.5.2, in the same manner as wild-type NDPK2 Arabidopsis thaliana
LP175S no interaction between NDPK2 and small G proteins, EC 3.6.5.2 Arabidopsis thaliana
P175S NDPK2 kinase site mutant Arabidopsis thaliana
P175S stimulates GTPase activites of Pra2 and Pra3, EC 3.6.5.2, with a slight reduction compared to wild-type NDPK2 Arabidopsis thaliana
R230stop C-terminal deletion mutant Arabidopsis thaliana
R230stop increased enzymatic activity, stimulates GTPase activites of Pra2 and Pra3, EC 3.6.5.2, in the same manner as wild-type NDPK2 Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Arabidopsis thaliana NDPK is involved in multiple signaling pathways in mammalian systems, including G-protein signaling. Physical interaction between NDPK2 and small G proteins, as well as the heterotrimeric G protein GPA1. NDPK2 can stimulate their GTPase activities, but the dimeric form of NDPK2 is also unable to enhance the GTPase activities of both Pra2 and Pra3, the role oligomeric states may play in the interaction of NDPK2 with small G proteins ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Arabidopsis thaliana O64903
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information NDPK is involved in multiple signaling pathways in mammalian systems, including G-protein signaling. Physical interaction between NDPK2 and small G proteins, as well as the heterotrimeric G protein GPA1. NDPK2 can stimulate their GTPase activities, but the dimeric form of NDPK2 is also unable to enhance the GTPase activities of both Pra2 and Pra3, the role oligomeric states may play in the interaction of NDPK2 with small G proteins Arabidopsis thaliana ?
-
 ?
additional information NDPK2 interacts with plant small G proteins in vitro, both Pra2 and Pra3both Pra2 and Pra3, the role oligomeric states may play in the interaction of NDPK2 with small G proteins Arabidopsis thaliana ?
-
 ?

Subunits

Subunits Comment Organism
hexamer
-
 Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
NDPK2
-
 Arabidopsis thaliana
nucleoside diphosphate kinase
-
 Arabidopsis thaliana
nucleoside diphosphate kinase-2
-
 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Arabidopsis thaliana