Cloned (Comment) | Organism |
---|---|
expressed as GST-fusion protein in Escherichia coli | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
H117A | no effect on nuclease activity | Mycobacterium tuberculosis |
K10A | nuclease activity completely abolished | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | inhibition of nuclease activity | Mycobacterium tuberculosis | |
GDP | competitive inhibition of nuclease activity | Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | enzyme is secreted into the culture medium | Mycobacterium tuberculosis | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for DNA cleavage reaction | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dNDP | Mycobacterium tuberculosis | contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates | ADP + dNTP | - |
? | |
ATP + dNDP | Mycobacterium tuberculosis H37Rv | contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates | ADP + dNTP | - |
? | |
ATP + NDP | Mycobacterium tuberculosis | contributes to the maintenance of the cellular pools of all nucleoside triphosphates, excreted enzyme enters the nucleus of HeLa and COS-1 cells and nicks chromosomal DNA in situ | ADP + NTP | - |
? | |
ATP + NDP | Mycobacterium tuberculosis H37Rv | contributes to the maintenance of the cellular pools of all nucleoside triphosphates, excreted enzyme enters the nucleus of HeLa and COS-1 cells and nicks chromosomal DNA in situ | ADP + NTP | - |
? | |
additional information | Mycobacterium tuberculosis | catalyzes cleavage of single strand DNA, oligonucleotide P1 used for experiments | ? | - |
? | |
additional information | Mycobacterium tuberculosis H37Rv | catalyzes cleavage of single strand DNA, oligonucleotide P1 used for experiments | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WJH7 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WJH7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein using GST-tag | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dNDP | contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates | Mycobacterium tuberculosis | ADP + dNTP | - |
? | |
ATP + dNDP | contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates | Mycobacterium tuberculosis H37Rv | ADP + dNTP | - |
? | |
ATP + GDP | - |
Mycobacterium tuberculosis | ADP + GTP | - |
? | |
ATP + GDP | - |
Mycobacterium tuberculosis H37Rv | ADP + GTP | - |
? | |
ATP + NDP | - |
Mycobacterium tuberculosis | ADP + NTP | - |
? | |
ATP + NDP | contributes to the maintenance of the cellular pools of all nucleoside triphosphates, excreted enzyme enters the nucleus of HeLa and COS-1 cells and nicks chromosomal DNA in situ | Mycobacterium tuberculosis | ADP + NTP | - |
? | |
ATP + NDP | - |
Mycobacterium tuberculosis H37Rv | ADP + NTP | - |
? | |
ATP + NDP | contributes to the maintenance of the cellular pools of all nucleoside triphosphates, excreted enzyme enters the nucleus of HeLa and COS-1 cells and nicks chromosomal DNA in situ | Mycobacterium tuberculosis H37Rv | ADP + NTP | - |
? | |
additional information | catalyzes cleavage of single strand DNA, oligonucleotide P1 used for experiments | Mycobacterium tuberculosis | ? | - |
? | |
additional information | catalyzes cleavage of single strand DNA, oligonucleotide P1 used for experiments | Mycobacterium tuberculosis H37Rv | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | - |
Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
GDP | competitive inhibition of nuclease activity | Mycobacterium tuberculosis |