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Literature summary for 2.7.4.6 extracted from

  • Kumar, P.; Verma, A.; Saini, A.K.; Chopra, P.; Chakraborti, P.K.; Singh, Y.; Chowdhury, S.
    Nucleoside diphosphate kinase from Mycobacterium tuberculosis cleaves single strand DNA within the human c-myc promoter in an enzyme-catalyzed reaction (2005), Nucleic Acids Res., 33, 2707-2714.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed as GST-fusion protein in Escherichia coli Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
H117A no effect on nuclease activity Mycobacterium tuberculosis
K10A nuclease activity completely abolished Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
ATP inhibition of nuclease activity Mycobacterium tuberculosis
GDP competitive inhibition of nuclease activity Mycobacterium tuberculosis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular enzyme is secreted into the culture medium Mycobacterium tuberculosis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for DNA cleavage reaction Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + dNDP Mycobacterium tuberculosis contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates ADP + dNTP
-
?
ATP + dNDP Mycobacterium tuberculosis H37Rv contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates ADP + dNTP
-
?
ATP + NDP Mycobacterium tuberculosis contributes to the maintenance of the cellular pools of all nucleoside triphosphates, excreted enzyme enters the nucleus of HeLa and COS-1 cells and nicks chromosomal DNA in situ ADP + NTP
-
?
ATP + NDP Mycobacterium tuberculosis H37Rv contributes to the maintenance of the cellular pools of all nucleoside triphosphates, excreted enzyme enters the nucleus of HeLa and COS-1 cells and nicks chromosomal DNA in situ ADP + NTP
-
?
additional information Mycobacterium tuberculosis catalyzes cleavage of single strand DNA, oligonucleotide P1 used for experiments ?
-
?
additional information Mycobacterium tuberculosis H37Rv catalyzes cleavage of single strand DNA, oligonucleotide P1 used for experiments ?
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WJH7
-
-
Mycobacterium tuberculosis H37Rv P9WJH7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein using GST-tag Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + dNDP contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates Mycobacterium tuberculosis ADP + dNTP
-
?
ATP + dNDP contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates Mycobacterium tuberculosis H37Rv ADP + dNTP
-
?
ATP + GDP
-
Mycobacterium tuberculosis ADP + GTP
-
?
ATP + GDP
-
Mycobacterium tuberculosis H37Rv ADP + GTP
-
?
ATP + NDP
-
Mycobacterium tuberculosis ADP + NTP
-
?
ATP + NDP contributes to the maintenance of the cellular pools of all nucleoside triphosphates, excreted enzyme enters the nucleus of HeLa and COS-1 cells and nicks chromosomal DNA in situ Mycobacterium tuberculosis ADP + NTP
-
?
ATP + NDP
-
Mycobacterium tuberculosis H37Rv ADP + NTP
-
?
ATP + NDP contributes to the maintenance of the cellular pools of all nucleoside triphosphates, excreted enzyme enters the nucleus of HeLa and COS-1 cells and nicks chromosomal DNA in situ Mycobacterium tuberculosis H37Rv ADP + NTP
-
?
additional information catalyzes cleavage of single strand DNA, oligonucleotide P1 used for experiments Mycobacterium tuberculosis ?
-
?
additional information catalyzes cleavage of single strand DNA, oligonucleotide P1 used for experiments Mycobacterium tuberculosis H37Rv ?
-
?

Subunits

Subunits Comment Organism
hexamer
-
Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.9
-
GDP competitive inhibition of nuclease activity Mycobacterium tuberculosis