Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Trimethylamine N-oxide | concentration of 4 M required for activity, at pH 8 inclusion of low concentrations of NaCl required for activation | Halobacterium salinarum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | concentration of 3 M required for activity | Halobacterium salinarum | |
NaCl | concentration of 2 M required for activity | Halobacterium salinarum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NDP | Halobacterium salinarum | contributes to the maintenance of the cellular pools of all nucleosides triphosphates | ADP + NTP | - |
? | |
ATP + NDP | Halobacterium salinarum 4R | contributes to the maintenance of the cellular pools of all nucleosides triphosphates | ADP + NTP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halobacterium salinarum | - |
- |
- |
Halobacterium salinarum 4R | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Halobacterium salinarum |
Renatured (Comment) | Organism |
---|---|
extremely halophilic enzyme inactivated at low salt concentrations using heat-treatment or 6 M urea, addition of or dialysis against 2 M NaCl, 3 M KCl, or 4 M trimethylamine N-oxide required for effective refolding of enzyme, refolding using 4 M trimethylamine N-oxide is strongly pH-dependent, good refolding at pH 7, almost no activity regained at pH 8, at pH 8 inclusion of low concentrations of NaCl supports refolding and activation of activity | Halobacterium salinarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NDP | - |
Halobacterium salinarum | ADP + NTP | - |
? | |
ATP + NDP | contributes to the maintenance of the cellular pools of all nucleosides triphosphates | Halobacterium salinarum | ADP + NTP | - |
? | |
ATP + NDP | - |
Halobacterium salinarum 4R | ADP + NTP | - |
? | |
ATP + NDP | contributes to the maintenance of the cellular pools of all nucleosides triphosphates | Halobacterium salinarum 4R | ADP + NTP | - |
? |