Cloned (Comment) | Organism |
---|---|
wild type and mutant proteins expressed as GST-fusion protein in Escherichia coli BL21(DE3) or DH5alpha, complementation studies with NdK deficient Pseudomonas aeruginosa | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
H52A | unable to complement NdK deficient Pseudomonas aeruginosa, Km values similar to wild type protein but significantly reduced turnover | Mycobacterium tuberculosis |
H53Q | unable to complement NdK deficient Pseudomonas aeruginosa, Km values similar to wild type protein but significantly reduced turnover | Mycobacterium tuberculosis |
K10A | no phosphotransfer activity | Mycobacterium tuberculosis |
K29A | no phosphotransfer activity | Mycobacterium tuberculosis |
R104A | no phosphotransfer activity | Mycobacterium tuberculosis |
R86A | no phosphotransfer activity | Mycobacterium tuberculosis |
Y50F | unable to complement NdK deficient Pseudomonas aeruginosa, no phosphotransfer activity | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | IC50: 1.9 mM | Mycobacterium tuberculosis | |
cAMP | IC50: 26.7 mM | Mycobacterium tuberculosis | |
chromoglycate | IC50: 90 mM | Mycobacterium tuberculosis | |
UDP | IC50: 1.6 mM | Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | enzyme is secreted to the culture medium | Mycobacterium tuberculosis | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | divalent cation required for catalysis | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16500 | - |
6 * 16500 | Mycobacterium tuberculosis |
107000 | - |
gel filtration | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dNDP | Mycobacterium tuberculosis | contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates | ADP + dNTP | - |
? | |
ATP + dNDP | Mycobacterium tuberculosis H37Ra / ATCC 25177 | contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates | ADP + dNTP | - |
? | |
ATP + NDP | Mycobacterium tuberculosis | contributes to the maintenance of the cellular pools of all nucleoside triphosphates | ADP + NTP | - |
? | |
ATP + NDP | Mycobacterium tuberculosis H37Ra / ATCC 25177 | contributes to the maintenance of the cellular pools of all nucleoside triphosphates | ADP + NTP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | A5U5E1 | - |
- |
Mycobacterium tuberculosis H37Ra / ATCC 25177 | A5U5E1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombisnt proteins using GST-tag | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + dGTP | - |
Mycobacterium tuberculosis | ATP + dGDP | - |
? | |
ADP + dGTP | - |
Mycobacterium tuberculosis H37Ra / ATCC 25177 | ATP + dGDP | - |
? | |
ATP + dNDP | contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates | Mycobacterium tuberculosis | ADP + dNTP | - |
? | |
ATP + dNDP | contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates | Mycobacterium tuberculosis H37Ra / ATCC 25177 | ADP + dNTP | - |
? | |
ATP + NDP | - |
Mycobacterium tuberculosis | ADP + NTP | - |
? | |
ATP + NDP | contributes to the maintenance of the cellular pools of all nucleoside triphosphates | Mycobacterium tuberculosis | ADP + NTP | - |
? | |
ATP + NDP | - |
Mycobacterium tuberculosis H37Ra / ATCC 25177 | ADP + NTP | - |
? | |
ATP + NDP | contributes to the maintenance of the cellular pools of all nucleoside triphosphates | Mycobacterium tuberculosis H37Ra / ATCC 25177 | ADP + NTP | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 16500 | Mycobacterium tuberculosis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
1.6 | - |
IC50: 1.6 mM | Mycobacterium tuberculosis | UDP | |
1.9 | - |
IC50: 1.9 mM | Mycobacterium tuberculosis | AMP | |
26.7 | - |
IC50: 26.7 mM | Mycobacterium tuberculosis | cAMP | |
90 | - |
IC50: 90 mM | Mycobacterium tuberculosis | chromoglycate |