Cloned (Comment) | Organism |
---|---|
native and mutant enzymes expressed as His-tag fusion protein in Escherichia coli SG13009 | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
H117Q | significant loss of activity with ATP and GTP | Mycobacterium tuberculosis |
H49Q | no effect on activity | Mycobacterium tuberculosis |
H53Q | no effect on activity | Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | enzyme is secreted to the culture medium | Mycobacterium tuberculosis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14400 | - |
x * 14400, SDS-PAGE | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NDP | Mycobacterium tuberculosis | contributes to the maintenance of the cellular pools of all nucleosides triphosphates, extracellular enzyme contributes to ATP-induced cell death of macrophages | ADP + NTP | - |
? | |
ATP + NDP | Mycobacterium tuberculosis H37Rv | contributes to the maintenance of the cellular pools of all nucleosides triphosphates, extracellular enzyme contributes to ATP-induced cell death of macrophages | ADP + NTP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzymes using His-tag | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NDP | - |
Mycobacterium tuberculosis | ADP + NTP | - |
? | |
ATP + NDP | contributes to the maintenance of the cellular pools of all nucleosides triphosphates, extracellular enzyme contributes to ATP-induced cell death of macrophages | Mycobacterium tuberculosis | ADP + NTP | - |
? | |
ATP + NDP | - |
Mycobacterium tuberculosis H37Rv | ADP + NTP | - |
? | |
ATP + NDP | contributes to the maintenance of the cellular pools of all nucleosides triphosphates, extracellular enzyme contributes to ATP-induced cell death of macrophages | Mycobacterium tuberculosis H37Rv | ADP + NTP | - |
? | |
GTP + NDP | - |
Mycobacterium tuberculosis | GDP + NTP | - |
? | |
GTP + NDP | - |
Mycobacterium tuberculosis H37Rv | GDP + NTP | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 14400, SDS-PAGE | Mycobacterium tuberculosis |