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Literature summary for 2.7.4.6 extracted from

  • Takeishi, S.; Nakagawa, N.; Maoka, N.; Kihara, M.; Moriguchi, M.; Masui, R.; Kuramitsu, S.
    Crystallization and preliminary X-ray diffraction studies of nucleoside diphosphate kinase from Thermus thermophilus HB8 (2003), Acta Crystallogr. Sect. D, 59, 1843-1845.
    View publication on PubMed
Search for more literature for 2.7.4.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysE Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 GDP pH 7.5, 25°C Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15000
-
 4 * 15000, calculated from the deduced amino acid sequence, SDS-PAGE, native mass by gel filtration Thermus thermophilus
60000
-
 gel filtration Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + NDP Thermus thermophilus contributes to the maintenance of the cellular pools of all nucleosides triphosphates ADP + NTP
-
 ?
ATP + NDP Thermus thermophilus HB8 / ATCC 27634 / DSM 579 contributes to the maintenance of the cellular pools of all nucleosides triphosphates ADP + NTP
-
 ?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Thermus thermophilus

Reaction

Reaction Comment Organism Reaction ID
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate two-step ping-pong mechanism Thermus thermophilus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + CDP
-
 Thermus thermophilus ADP + CTP
-
 ?
ATP + CDP
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ADP + CTP
-
 ?
ATP + GDP
-
 Thermus thermophilus ADP + GTP
-
 ?
ATP + GDP
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ADP + GTP
-
 ?
ATP + NDP
-
 Thermus thermophilus ADP + NTP
-
 ?
ATP + NDP contributes to the maintenance of the cellular pools of all nucleosides triphosphates Thermus thermophilus ADP + NTP
-
 ?
ATP + NDP
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ADP + NTP
-
 ?
ATP + NDP contributes to the maintenance of the cellular pools of all nucleosides triphosphates Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ADP + NTP
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 15000, calculated from the deduced amino acid sequence, SDS-PAGE, native mass by gel filtration Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
38
-
 GDP pH 7.5, 25 °C Thermus thermophilus