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Literature summary for 2.7.4.6 extracted from
- Purification and characterization of nucleoside diphosphate kinase from spinach leaves (1991), Biochim. Biophys. Acta, 1077, 47-55.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| GDP | at higher concentrations, substrate inhibition | Spinacia oleracea |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.091 | 0.1 | GDP | cosubstrate ATP, NDP-kinase I and II, 37°C, pH 7.4 | Spinacia oleracea | |
| 0.89 | - |
ATP | cosubstrate GDP, NDP-kinase II, 37°C, pH 7.4 | Spinacia oleracea | |
| 2 | - |
ATP | NDP-kinase I, 37°C, pH 7.4 | Spinacia oleracea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Spinacia oleracea | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition | Spinacia oleracea |
| 16000 | - |
6 * 16000, isozyme I, SDS-PAGE | Spinacia oleracea |
| 18000 | - |
6 * 18000, SDS-PAGE, isozyme II | Spinacia oleracea |
| 92000 | - |
isozyme I, gel filtration | Spinacia oleracea |
| 110000 | - |
isozyme II, gel filtration | Spinacia oleracea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2 isozymes: NDP-kinase I and II | Spinacia oleracea |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + nucleoside diphosphate = ADP + nucleoside triphosphate | the catalytic mechanism involves a phosphoenzyme intermediate | Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1250 | - |
NDP-kinase I, 37°C, pH 7.4 | Spinacia oleracea |
| 2160 | - |
NDP-kinase II, 37°C, pH 7.4 | Spinacia oleracea |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 0.05 M 2-(N-morpholino)ethanesulfonic acid, pH 5.5, 1 mM EDTA, 0.2 mM PMSF, 0.1 M NaCl, 5 mM 2-mercaptoethanol, 0.1% Lubrol, several months | Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| gamma-S-ATP + GDP | poor substrate | Spinacia oleracea | ADP + gamma-S-GTP | - |
? | |
| gamma-S-GTP + GDP | poor substrate | Spinacia oleracea | GDP + gamma-S-GTP | - |
? | |
| GTP + ADP | - |
Spinacia oleracea | GDP + ATP | - |
r | |
| NTP + nucleoside diphosphate | - |
Spinacia oleracea | NDP + NTP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 16000, isozyme I, SDS-PAGE | Spinacia oleracea |
| hexamer | 6 * 18000, SDS-PAGE, isozyme II | Spinacia oleracea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Spinacia oleracea |
| 37 | - |
assay at | Spinacia oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Spinacia oleracea |
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