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Literature summary for 2.7.4.6 extracted from
- Nucleoside diphosphokinase from beef heart cytosol. I. Physical and kinetic properties (1972), Biochemistry, 11, 3370-3378.
General Stability
| General Stability | Organism |
|---|---|
| unstable in dilute solutions, bovine serum albumin stabilizes, not DTT | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | kinetics, substrate inhibition | Bos taurus |  |
| MgADP- | in excess | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | effect of Mg2+ on kinetic parameters | Bos taurus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Bos taurus | 5829 | - |
| mitochondrion | dual cellular localization in mitochondria and cytosol | Bos taurus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Bos taurus | |
| Mg2+ | true substrates: Mg-ADP and Mg-ATP | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 103000 | - |
sucrose density gradient centrifugation | Bos taurus |
| 108000 | - |
gel filtration | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + nucleoside diphosphate = ADP + nucleoside triphosphate | mechanism | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 360 | - |
25°C, pH 8.0 | Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-2°C, 2 mg bovine serum albumin/ml, 0.04% NaN3, at least 1 month | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + adenosine 5'-methylene diphosphonate | - |
Bos taurus | ADP + ? | - |
? | |
| ATP + ADP | best substrate | Bos taurus | ADP + ATP | - |
? | |
| ATP + CDP | reverse reaction at 21% the rate of ATP plus ADP | Bos taurus | ADP + CTP | - |
r | |
| ATP + UDP | reverse reaction at 32% the rate of the reaction ATP plus ADP | Bos taurus | ADP + UTP | - |
r | |
| CTP + CDP | reaction at 15% the rate of ATP | Bos taurus | CDP + CTP | - |
? | |
| GTP + ADP | reaction at 12% the rate of ATP | Bos taurus | GDP + ATP | - |
? | |
| NTP + nucleoside diphosphate | the nature of the nucleoside triphosphate controls the rate of the reaction | Bos taurus | NDP + NTP | - |
? | |
| UTP + UDP | reaction at 31% the rate of ATP | Bos taurus | UDP + UTP | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
1 h, 0.0018 mg enzyme/ml, in 0.1 M phosphate buffer, pH 7, more than 50% loss of activity, 2 mg/ml bovine serum albumin plus 2 mM Mg-ADP or 5 mM free ADP protect, not MgCl2 or free ADP alone | Bos taurus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
inactivation below | Bos taurus |
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