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Literature summary for 2.7.4.4 extracted from

  • Strominger, J.L.; Heppel, L.A.; Maxwell, E.S.
    Nucleoside monophosphate kinases. Transphosphorilation between adenosine triphosphate and nucleoside monophosphates (1959), Biochim. Biophys. Acta, 32, 412-421.
    View publication on PubMed
Search for more literature for 2.7.4.4

Activating Compound

Activating Compound Comment Organism Structure
EDTA variable degree of activation Bos taurus
glutathione variable degree of activation Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ half as effective as Mg2+ at 4 mM Bos taurus
Mg2+ activity saturation at 4 mM Bos taurus
Mn2+ as effective as Mg2+ Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, using ammonium sulfate precipitation Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 description of assay method Bos taurus

Storage Stability

Storage Stability Organism
-15°C, 3 weeks, 75% of its activity lost Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + UMP
-
 Bos taurus ADP + UDP
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 completely inactivated at pH 7 Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 7.5 10% of the activity at pH 5.3 Bos taurus

pH Stability

pH Stability pH Stability Maximum Comment Organism
1.3
-
 inactivation in few min at 2°C Bos taurus