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Literature summary for 2.7.4.33 extracted from

  • Jyoti Roy, J.; Mondal, S.; Bera, T.
    Polyphosphate kinase from Leishmania donovani amastigotes: A polyphosphate driven generator of ATP (2014), Int. J. Drug Develop. Res., 6, 159-164.
No PubMed abstract available

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Leishmania donovani

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + (phosphate)n Leishmania donovani cf. EC 2.7.4.1 ATP + (phosphate)n-1
-
r
ADP + (phosphate)n Leishmania donovani MHOM/IN/83/AG83 cf. EC 2.7.4.1 ATP + (phosphate)n-1
-
r
AMP + (phosphate)n Leishmania donovani
-
ADP + (phosphate)n-1
-
r

Organism

Organism UniProt Comment Textmining
Leishmania donovani
-
stibogluconate-resistant and paromomycin-resistant clone AG83
-
Leishmania donovani MHOM/IN/83/AG83
-
stibogluconate-resistant and paromomycin-resistant clone AG83
-

Source Tissue

Source Tissue Comment Organism Textmining
amastigote
-
Leishmania donovani
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0002
-
ATP formation from AMP, pH 7.0, 37°C, PMM-resistant clone AG83 Leishmania donovani
0.00047
-
ATP formation from AMP, pH 7.0, 37°C, wild-type clone AG83 Leishmania donovani
0.0005
-
ATP formation from AMP, pH 7.0, 37°C, SSG-resistant clone AG83 Leishmania donovani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + (phosphate)n cf. EC 2.7.4.1 Leishmania donovani ATP + (phosphate)n-1
-
r
ADP + (phosphate)n cf. EC 2.7.4.1 Leishmania donovani MHOM/IN/83/AG83 ATP + (phosphate)n-1
-
r
AMP + (phosphate)n
-
Leishmania donovani ADP + (phosphate)n-1
-
r
additional information maximum PPK activity is observed for wild-type AG83 Leishmania donovani amastigotes when ADP is the phosphate group acceptor from polyphosphate, the activity is 29 times higher than with GDP as phosphate group acceptor from polyphosphate. When AMP is the phosphate group acceptor from polyphosphate, the activity of PPK is 97times less than with ADP, existence of both PPK1 and PPK2 activity is probable in Leishmania donovani amastigotes. ATP formation from AMP shows lowest activity Leishmania donovani ?
-
?
additional information maximum PPK activity is observed for wild-type AG83 Leishmania donovani amastigotes when ADP is the phosphate group acceptor from polyphosphate, the activity is 29 times higher than with GDP as phosphate group acceptor from polyphosphate. When AMP is the phosphate group acceptor from polyphosphate, the activity of PPK is 97times less than with ADP, existence of both PPK1 and PPK2 activity is probable in Leishmania donovani amastigotes. ATP formation from AMP shows lowest activity Leishmania donovani MHOM/IN/83/AG83 ?
-
?

Synonyms

Synonyms Comment Organism
PPK
-
Leishmania donovani

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Leishmania donovani

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Leishmania donovani

General Information

General Information Comment Organism
physiological function substrate level phosphorylation is essential for the survival of amastigote forms of Leishmania donovani Leishmania donovani