Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Leishmania donovani |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + (phosphate)n | Leishmania donovani | cf. EC 2.7.4.1 | ATP + (phosphate)n-1 | - |
r | |
ADP + (phosphate)n | Leishmania donovani MHOM/IN/83/AG83 | cf. EC 2.7.4.1 | ATP + (phosphate)n-1 | - |
r | |
AMP + (phosphate)n | Leishmania donovani | - |
ADP + (phosphate)n-1 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | - |
stibogluconate-resistant and paromomycin-resistant clone AG83 | - |
Leishmania donovani MHOM/IN/83/AG83 | - |
stibogluconate-resistant and paromomycin-resistant clone AG83 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
amastigote | - |
Leishmania donovani | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0002 | - |
ATP formation from AMP, pH 7.0, 37°C, PMM-resistant clone AG83 | Leishmania donovani |
0.00047 | - |
ATP formation from AMP, pH 7.0, 37°C, wild-type clone AG83 | Leishmania donovani |
0.0005 | - |
ATP formation from AMP, pH 7.0, 37°C, SSG-resistant clone AG83 | Leishmania donovani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + (phosphate)n | cf. EC 2.7.4.1 | Leishmania donovani | ATP + (phosphate)n-1 | - |
r | |
ADP + (phosphate)n | cf. EC 2.7.4.1 | Leishmania donovani MHOM/IN/83/AG83 | ATP + (phosphate)n-1 | - |
r | |
AMP + (phosphate)n | - |
Leishmania donovani | ADP + (phosphate)n-1 | - |
r | |
additional information | maximum PPK activity is observed for wild-type AG83 Leishmania donovani amastigotes when ADP is the phosphate group acceptor from polyphosphate, the activity is 29 times higher than with GDP as phosphate group acceptor from polyphosphate. When AMP is the phosphate group acceptor from polyphosphate, the activity of PPK is 97times less than with ADP, existence of both PPK1 and PPK2 activity is probable in Leishmania donovani amastigotes. ATP formation from AMP shows lowest activity | Leishmania donovani | ? | - |
? | |
additional information | maximum PPK activity is observed for wild-type AG83 Leishmania donovani amastigotes when ADP is the phosphate group acceptor from polyphosphate, the activity is 29 times higher than with GDP as phosphate group acceptor from polyphosphate. When AMP is the phosphate group acceptor from polyphosphate, the activity of PPK is 97times less than with ADP, existence of both PPK1 and PPK2 activity is probable in Leishmania donovani amastigotes. ATP formation from AMP shows lowest activity | Leishmania donovani MHOM/IN/83/AG83 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PPK | - |
Leishmania donovani |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Leishmania donovani |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Leishmania donovani |
General Information | Comment | Organism |
---|---|---|
physiological function | substrate level phosphorylation is essential for the survival of amastigote forms of Leishmania donovani | Leishmania donovani |