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Literature summary for 2.7.4.3 extracted from
- Analysis of thermal stabilizing interactions in mesophilic and thermophilic adenylate kinases from the genus Methanococcus (1999), J. Biol. Chem., 274, 28453-28458.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Methanothermococcus thermolithotrophicus |
| overexpression in Escherichia coli | Methanotorris igneus |
| overexpression in Escherichia coli, wild-type enzyme, mutant enzymes and chimeric enzymes | Methanocaldococcus jannaschii |
| overexpression in Escherichia colii, wild-type enzyme, mutant enzymes and chimeric enzymes | Methanococcus voltae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | P43409 | - |
- |
| Methanocaldococcus jannaschii DSM 2661 | P43409 | - |
- |
| Methanococcus voltae | P43411 | - |
- |
| Methanothermococcus thermolithotrophicus | - |
- |
- |
| Methanotorris igneus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanothermococcus thermolithotrophicus |
- |
Methanococcus voltae |
- |
Methanocaldococcus jannaschii |
- |
Methanotorris igneus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + AMP | - |
Methanothermococcus thermolithotrophicus | 2 ADP | - |
? |  |
| ATP + AMP | - |
Methanococcus voltae | 2 ADP | - |
? | |
| ATP + AMP | - |
Methanocaldococcus jannaschii | 2 ADP | - |
? | |
| ATP + AMP | - |
Methanotorris igneus | 2 ADP | - |
? | |
| ATP + AMP | - |
Methanocaldococcus jannaschii DSM 2661 | 2 ADP | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
wild-type enzyme | Methanococcus voltae |
| 45 | - |
mutant enzyme J36V | Methanococcus voltae |
| 53 | - |
mutant enzyme V160J | Methanococcus voltae |
| 68 | - |
wild-type enzyme | Methanothermococcus thermolithotrophicus |
| 72 | - |
mutant enzyme V36J | Methanocaldococcus jannaschii |
| 75 | - |
mutant enzyme J160V | Methanocaldococcus jannaschii |
| 80 | - |
wild-type enzyme | Methanotorris igneus |
| 83 | - |
wild-type enzyme | Methanocaldococcus jannaschii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 60 | 25°C: about 85% of maximal activity, 60°C: about 60% of maximal activity, wilde-type enzyme | Methanococcus voltae |
| 60 | 90 | 60°C: about 50% of amximal activity, 90°C: about 89% of maximal activity | Methanocaldococcus jannaschii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 69 | - |
Tm-value, wild-type enzyme | Methanococcus voltae |
| 73 | - |
Tm-value, mutant enzyme J36V | Methanococcus voltae |
| 74 | - |
Tm-value, mutant enzyme V160J | Methanococcus voltae |
| 86 | - |
Tm-value, wild-type enzyme | Methanothermococcus thermolithotrophicus |
| 95 | - |
tm-value is above 95°C | Methanotorris igneus |
| 96 | - |
Tm-value, mutant enzyme V160J | Methanocaldococcus jannaschii |
| 98 | - |
Tm-value, mutant enzyme J36V | Methanocaldococcus jannaschii |
| 103 | - |
Tm-value, wild-type enzyme | Methanocaldococcus jannaschii |
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