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Literature summary for 2.7.4.3 extracted from

  • Marques, I.d.e..A.; Romanello, L.; DeMarco, R.; Pereira, H.D.
    Structural and kinetic studies of Schistosoma mansoni adenylate kinases (2012), Mol. Biochem. Parasitol., 185, 157-160.
    View publication on PubMed
Search for more literature for 2.7.4.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Schistosoma mansoni

Crystallization (Commentary)

Crystallization (Comment) Organism
apo isoform ADK1, hanging drop vapor diffusion method, using 100 mM Tris-HCl (pH 8.5) and 2.4 M dibasic ammonium phosphate Schistosoma mansoni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
 ATP isoform ADK2, in 20 mM Tris-HCl (pH 7.4), 150 mM NaCl and 5 mM 2-mercaptoethanol, temperature not specified in the publication Schistosoma mansoni
0.037
-
 AMP isoform ADK2, in 20 mM Tris-HCl (pH 7.4), 150 mM NaCl and 5 mM 2-mercaptoethanol, temperature not specified in the publication Schistosoma mansoni
0.244
-
 AMP isoform ADK1, in 20 mM Tris-HCl (pH 7.4), 150 mM NaCl and 5 mM 2-mercaptoethanol, temperature not specified in the publication Schistosoma mansoni
0.272
-
 ATP isoform ADK1, in 20 mM Tris-HCl (pH 7.4), 150 mM NaCl and 5 mM 2-mercaptoethanol, temperature not specified in the publication Schistosoma mansoni

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Schistosoma mansoni 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22300
-
 1 * 22300, isoform ADK1, calculated from amino acid sequence Schistosoma mansoni
27200
-
 1 * 27200, isoform ADK2, calculated from amino acid sequence Schistosoma mansoni

Organism

Organism UniProt Comment Textmining
Schistosoma mansoni
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography and Superdex 200 gel filtration Schistosoma mansoni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + AMP
-
 Schistosoma mansoni ADP + ADP
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 22300, isoform ADK1, calculated from amino acid sequence Schistosoma mansoni
monomer 1 * 27200, isoform ADK2, calculated from amino acid sequence Schistosoma mansoni

Synonyms

Synonyms Comment Organism
ADK
-
 Schistosoma mansoni
ADK1 isoform Schistosoma mansoni
ADK2 isoform Schistosoma mansoni

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5
-
 ATP isoform ADK2, in 20 mM Tris-HCl (pH 7.4), 150 mM NaCl and 5 mM 2-mercaptoethanol, temperature not specified in the publication Schistosoma mansoni
8.3
-
 AMP isoform ADK2, in 20 mM Tris-HCl (pH 7.4), 150 mM NaCl and 5 mM 2-mercaptoethanol, temperature not specified in the publication Schistosoma mansoni
25
-
 ATP isoform ADK1, in 20 mM Tris-HCl (pH 7.4), 150 mM NaCl and 5 mM 2-mercaptoethanol, temperature not specified in the publication Schistosoma mansoni
50
-
 AMP isoform ADK1, in 20 mM Tris-HCl (pH 7.4), 150 mM NaCl and 5 mM 2-mercaptoethanol, temperature not specified in the publication Schistosoma mansoni

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2600
-
 ATP isoform ADK2, in 20 mM Tris-HCl (pH 7.4), 150 mM NaCl and 5 mM 2-mercaptoethanol, temperature not specified in the publication Schistosoma mansoni
4500
-
 AMP isoform ADK2, in 20 mM Tris-HCl (pH 7.4), 150 mM NaCl and 5 mM 2-mercaptoethanol, temperature not specified in the publication Schistosoma mansoni
4900
-
 AMP isoform ADK1, in 20 mM Tris-HCl (pH 7.4), 150 mM NaCl and 5 mM 2-mercaptoethanol, temperature not specified in the publication Schistosoma mansoni
11000
-
 ATP isoform ADK1, in 20 mM Tris-HCl (pH 7.4), 150 mM NaCl and 5 mM 2-mercaptoethanol, temperature not specified in the publication Schistosoma mansoni