Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.4.3 extracted from

  • Krishnamurthy, H.; Munro, K.; Yan, H.; Vieille, C.
    Dynamics in Thermotoga neapolitana adenylate kinase: 15N relaxation and hydrogen-deuterium exchange studies of a hyperthermophilic enzyme highly active at 30°C (2009), Biochemistry, 48, 2723-2739.
    View publication on PubMed
Search for more literature for 2.7.4.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thermotoga neapolitana

Inhibitors

Inhibitors Comment Organism Structure
P1,P5-di(adenosine-5') pentaphosphate
-
 Escherichia coli
P1,P5-di(adenosine-5') pentaphosphate
-
 Thermotoga neapolitana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Escherichia coli
Mg2+ required for activity Thermotoga neapolitana

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Thermotoga neapolitana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation and Q-Sepharose column chromatography Escherichia coli
ammonium sulfate fractionation and Q-Sepharose column chromatography Thermotoga neapolitana

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + AMP
-
 Escherichia coli ADP + ADP
-
 r
ATP + AMP
-
 Thermotoga neapolitana ADP + ADP
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 highly active at 30°C Escherichia coli
30
-
 highly active at 30°C Thermotoga neapolitana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
52
-
 the melting temperature of the free enzyme is at 52°C Escherichia coli
99
-
 the melting temperature of the free enzyme is at 99°C Thermotoga neapolitana