Protein Variants | Comment | Organism |
---|---|---|
Q199R | modest increase in stability, 3 degrees increase in melting temperature. At temperatures of 20°C to 45°C, 50% loss of activity, with subsequent increase at higher temperatures. rigidification of he overall structure through stabilization of a polypeptide loop containing R199 that is part of the ATP-binding site | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
AMP | wild-type, pH 7.2, 27°C | Bacillus subtilis | |
0.013 | - |
AMP | mutant Q199R, pH 7.2, 27°C | Bacillus subtilis | |
0.016 | - |
AMP | wild-type, pH 7.2, 35°C | Bacillus subtilis | |
0.024 | - |
AMP | mutant Q199R, pH 7.2, 35°C | Bacillus subtilis | |
0.026 | - |
AMP | wild-type, pH 7.2, 45°C | Bacillus subtilis | |
0.046 | - |
AMP | mutant Q199R, pH 7.2, 45°C | Bacillus subtilis | |
0.048 | - |
AMP | wild-type, pH 7.2, 55°C | Bacillus subtilis | |
0.07 | - |
AMP | wild-type, pH 7.2, 60°C | Bacillus subtilis | |
0.094 | - |
AMP | mutant Q199R, pH 7.2, 55°C | Bacillus subtilis | |
0.11 | - |
AMP | mutant Q199R, pH 7.2, 60°C | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P16304 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
AMP + ATP | - |
Bacillus subtilis | ADP | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
AMP | mutant Q199R, pH 7.2, 27°C | Bacillus subtilis | |
0.035 | - |
AMP | mutant Q199R, pH 7.2, 35°C | Bacillus subtilis | |
0.035 | - |
AMP | wild-type, pH 7.2, 27°C | Bacillus subtilis | |
0.053 | - |
AMP | wild-type, pH 7.2, 35°C | Bacillus subtilis | |
0.06 | - |
AMP | mutant Q199R, pH 7.2, 45°C | Bacillus subtilis | |
0.073 | - |
AMP | wild-type, pH 7.2, 45°C | Bacillus subtilis | |
0.1 | - |
AMP | wild-type, pH 7.2, 55°C | Bacillus subtilis | |
0.13 | - |
AMP | mutant Q199R, pH 7.2, 55°C | Bacillus subtilis | |
0.135 | - |
AMP | wild-type, pH 7.2, 60°C | Bacillus subtilis | |
0.19 | - |
AMP | mutant Q199R, pH 7.2, 60°C | Bacillus subtilis |