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Literature summary for 2.7.4.3 extracted from

  • Counago, R.; Wilson, C.J.; Pena, M.I.; Wittung-Stafshede, P.; Shamoo, Y.
    An adaptive mutation in adenylate kinase that increases organismal fitness is linked to stability-activity trade-offs (2008), Protein Eng. Des. Sel., 21, 19-27.
    View publication on PubMed
Search for more literature for 2.7.4.3

Protein Variants

Protein Variants Comment Organism
Q199R modest increase in stability, 3 degrees increase in melting temperature. At temperatures of 20°C to 45°C, 50% loss of activity, with subsequent increase at higher temperatures. rigidification of he overall structure through stabilization of a polypeptide loop containing R199 that is part of the ATP-binding site Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 AMP wild-type, pH 7.2, 27°C Bacillus subtilis
0.013
-
 AMP mutant Q199R, pH 7.2, 27°C Bacillus subtilis
0.016
-
 AMP wild-type, pH 7.2, 35°C Bacillus subtilis
0.024
-
 AMP mutant Q199R, pH 7.2, 35°C Bacillus subtilis
0.026
-
 AMP wild-type, pH 7.2, 45°C Bacillus subtilis
0.046
-
 AMP mutant Q199R, pH 7.2, 45°C Bacillus subtilis
0.048
-
 AMP wild-type, pH 7.2, 55°C Bacillus subtilis
0.07
-
 AMP wild-type, pH 7.2, 60°C Bacillus subtilis
0.094
-
 AMP mutant Q199R, pH 7.2, 55°C Bacillus subtilis
0.11
-
 AMP mutant Q199R, pH 7.2, 60°C Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P16304
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AMP + ATP
-
 Bacillus subtilis ADP
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.015
-
 AMP mutant Q199R, pH 7.2, 27°C Bacillus subtilis
0.035
-
 AMP mutant Q199R, pH 7.2, 35°C Bacillus subtilis
0.035
-
 AMP wild-type, pH 7.2, 27°C Bacillus subtilis
0.053
-
 AMP wild-type, pH 7.2, 35°C Bacillus subtilis
0.06
-
 AMP mutant Q199R, pH 7.2, 45°C Bacillus subtilis
0.073
-
 AMP wild-type, pH 7.2, 45°C Bacillus subtilis
0.1
-
 AMP wild-type, pH 7.2, 55°C Bacillus subtilis
0.13
-
 AMP mutant Q199R, pH 7.2, 55°C Bacillus subtilis
0.135
-
 AMP wild-type, pH 7.2, 60°C Bacillus subtilis
0.19
-
 AMP mutant Q199R, pH 7.2, 60°C Bacillus subtilis