Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | no inhibitory up to 150 mM, 65% residual activity at 700 mM | Desulfovibrio desulfuricans | |
KCl | not inhibitory up to 150 mM, 65% residual activity at 700 mM | Megalodesulfovibrio gigas |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cobalt | 0.3 mol of cobalt and 0.1 mol of zinc per mol of protein | Desulfovibrio desulfuricans | |
Cobalt | 0.4 mol of cobalt and 0.3 mol of zinc per mol of protein. Presence of three sulfydryl groups of cysteines potentially bound to Co2+ or Zn2+. Bound Zn2+ or Co2+ is clearly present in the LID domain and tetrahedrally coordinated to 129Cys, 135His, 151Cys, and 154Cys. Site 129Cys-X5-His-X15-Cys-X2-Cys is responsible for chelating zinc or cobalt | Megalodesulfovibrio gigas | |
Zinc | 0.3 mol of cobalt and 0.1 mol of zinc per mol of protein | Desulfovibrio desulfuricans | |
Zinc | 0.4 mol of cobalt and 0.3 mol of zinc per mol of protein. Presence of three sulfydryl groups of cysteines potentially bound to Co2+ or Zn2+. Bound Zn2+ or Co2+ is clearly present in the LID domain and tetrahedrally coordinated to 129Cys, 135His, 151Cys, and 154Cys. Site 129Cys-X5-His-X15-Cys-X2-Cys is responsible for chelating zinc or cobalt | Megalodesulfovibrio gigas |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24500 | - |
1 * 24500, SDS-PAGE, 1 * 24700, electrospray mass spectrometry, 1 * 24500, calculated | Megalodesulfovibrio gigas |
24700 | - |
1 * 24500, SDS-PAGE, 1 * 24700, electrospray mass spectrometry, 1 * 24500, calculated | Megalodesulfovibrio gigas |
24800 | - |
1 * 25500, SDS-PAGE, 1 * 24800, electrospray mass spectrometry | Desulfovibrio desulfuricans |
25500 | - |
1 * 25500, SDS-PAGE, 1 * 24800, electrospray mass spectrometry | Desulfovibrio desulfuricans |
26000 | - |
gel filtration | Desulfovibrio desulfuricans |
26000 | - |
gel filtration | Megalodesulfovibrio gigas |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desulfovibrio desulfuricans | - |
- |
- |
Megalodesulfovibrio gigas | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1560 | - |
apoenzyme, pH 7.4, 30°C | Megalodesulfovibrio gigas |
1640 | - |
ATP formation, pH 7.4, 30°C | Desulfovibrio desulfuricans |
1980 | - |
fully zinc-substitued form, pH 7.4, 30°C | Megalodesulfovibrio gigas |
2020 | - |
native form, pH 7.4, 30°C | Megalodesulfovibrio gigas |
2170 | - |
fully cobalt-substitued form, pH 7.4, 30°C | Megalodesulfovibrio gigas |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP | - |
Desulfovibrio desulfuricans | AMP + ATP | - |
r | |
ADP | - |
Megalodesulfovibrio gigas | AMP + ATP | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 24500, SDS-PAGE, 1 * 24700, electrospray mass spectrometry, 1 * 24500, calculated | Megalodesulfovibrio gigas |
monomer | 1 * 25500, SDS-PAGE, 1 * 24800, electrospray mass spectrometry | Desulfovibrio desulfuricans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
ADP formation | Desulfovibrio desulfuricans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.8 | 6.5 | direction of ADP formation | Megalodesulfovibrio gigas |
7.5 | 9 | direction of ATP formation | Megalodesulfovibrio gigas |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.8 | 11 | stable within | Desulfovibrio desulfuricans |
4.8 | 11 | stable within | Megalodesulfovibrio gigas |