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Literature summary for 2.7.4.3 extracted from
- Adenylate kinase from Pseudomonas denitrificans. I. Purification and antiserum inhibition (1974), J. Biochem., 75, 1027-1036.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag2+ | - |
Homo sapiens |  |
| Ag2+ | - |
Oryctolagus cuniculus | |
| Ag2+ | - |
Pseudomonas denitrificans (nom. rej.) | |
| AMP | - |
Bos taurus | |
| AMP | - |
Homo sapiens | |
| AMP | - |
Oryctolagus cuniculus | |
| AMP | - |
Pseudomonas denitrificans (nom. rej.) | |
| F- | not | Bos taurus | |
| F- | not | Homo sapiens | |
| F- | not | Oryctolagus cuniculus | |
| F- | not | Pseudomonas denitrificans (nom. rej.) | |
| Hg2+ | - |
Pseudomonas denitrificans (nom. rej.) | |
| homologous antibodies | - |
Bos taurus | |
| homologous antibodies | - |
Homo sapiens | |
| homologous antibodies | - |
Oryctolagus cuniculus | |
| homologous antibodies | - |
Pseudomonas denitrificans (nom. rej.) | |
| IAA | not | Bos taurus | |
| IAA | not | Homo sapiens | |
| IAA | not | Pseudomonas denitrificans (nom. rej.) | |
| additional information | not inhibitory: p-chloromercuribenzoate | Bos taurus | |
| additional information | not inhibitory: p-chloromercuribenzoate | Homo sapiens | |
| additional information | not inhibitory: p-chloromercuribenzoate | Oryctolagus cuniculus | |
| additional information | not inhibitory: p-chloromercuribenzoate | Pseudomonas denitrificans (nom. rej.) | |
| additional information | - |
Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic constants of adenylate kinases from various sources | Homo sapiens | |
| additional information | - |
additional information | kinetic constants of adenylate kinases from various sources | Rattus norvegicus | |
| additional information | - |
additional information | kinetic constants of adenylate kinases from various sources | Bos taurus | |
| additional information | - |
additional information | kinetic constants of adenylate kinases from various sources | Oryctolagus cuniculus | |
| 0.24 | 0.27 | ADP | - |
Homo sapiens | |
| 0.24 | 0.27 | ADP | - |
Bos taurus | |
| 0.24 | 0.27 | ADP | - |
Oryctolagus cuniculus | |
| 0.24 | 0.27 | ADP | 25°C, pH 7.6 | Pseudomonas denitrificans (nom. rej.) | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
molecular weights of enzymes from different organisms | Homo sapiens |
| additional information | - |
molecular weights of enzymes from different organisms | Bos taurus |
| additional information | - |
molecular weights of enzymes from different organisms | Oryctolagus cuniculus |
| additional information | - |
molecular weights of enzymes from different organisms | Pseudomonas denitrificans (nom. rej.) |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Oryctolagus cuniculus | - |
- |
- |
| Pseudomonas denitrificans (nom. rej.) | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, to near homogeneity | Pseudomonas denitrificans (nom. rej.) |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bos taurus |
| additional information | - |
- |
Oryctolagus cuniculus |
| additional information | - |
- |
Pseudomonas denitrificans (nom. rej.) |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 30% or 70% loss of activity at pH 6 or pH 5, respectively, on standing overnight | Pseudomonas denitrificans (nom. rej.) |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + ADP | - |
Homo sapiens | ATP + AMP | - |
r | |
| ADP + ADP | - |
Rattus norvegicus | ATP + AMP | - |
r | |
| ADP + ADP | - |
Bos taurus | ATP + AMP | - |
r | |
| ADP + ADP | - |
Oryctolagus cuniculus | ATP + AMP | - |
r | |
| ADP + ADP | - |
Pseudomonas denitrificans (nom. rej.) | ATP + AMP | - |
r | |
| ATP + AMP | - |
Homo sapiens | ADP + ADP | - |
r | |
| ATP + AMP | - |
Rattus norvegicus | ADP + ADP | - |
r | |
| ATP + AMP | - |
Bos taurus | ADP + ADP | - |
r | |
| ATP + AMP | - |
Oryctolagus cuniculus | ADP + ADP | - |
r | |
| ATP + AMP | - |
Pseudomonas denitrificans (nom. rej.) | ADP + ADP | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 8.6 | broad | Pseudomonas denitrificans (nom. rej.) |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | 70% loss of activity at pH 5 in 50 mM acetate buffer and 30% loss of activity at pH 6 in phosphate buffer, at 4°C overnight | Homo sapiens |
| 5 | 6 | 70% loss of activity at pH 5 in 50 mM acetate buffer and 30% loss of activity at pH 6 in phosphate buffer, at 4°C overnight | Bos taurus |
| 5 | 6 | 70% loss of activity at pH 5 in 50 mM acetate buffer and 30% loss of activity at pH 6 in phosphate buffer, at 4°C overnight | Oryctolagus cuniculus |
| 5 | 6 | 70% loss of activity at pH 5 in 50 mM acetate buffer and 30% loss of activity at pH 6 in phosphate buffer, at 4°C overnight | Pseudomonas denitrificans (nom. rej.) |
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