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Literature summary for 2.7.4.3 extracted from

  • Neufang, H.; Müller, H.; Knobloch, K.
    Purification and properties of the adenylate kinases from Rhodopseudomonas palustris, Rhodopseudomonas shaeroides and Rhodopseudomonas rubrum (1983), Arch. Microbiol., 134, 153-157.
No PubMed abstract available
Search for more literature for 2.7.4.3

General Stability

General Stability Organism
stable to repeated freeze-thaw cycles, at 5-10 mg/ml Cereibacter sphaeroides
stable to repeated freeze-thaw cycles, at 5-10 mg/ml Rhodopseudomonas palustris
stable to repeated freeze-thaw cycles, at 5-10 mg/ml Rhodopseudomonas rubrum

Inhibitors

Inhibitors Comment Organism Structure
ADP in excess, substrate inhibition Cereibacter sphaeroides
ADP in excess, substrate inhibition Rhodopseudomonas palustris
ADP in excess, substrate inhibition Rhodopseudomonas rubrum
AMP product inhibition Cereibacter sphaeroides
AMP product inhibition Rhodopseudomonas palustris
AMP product inhibition Rhodopseudomonas rubrum
F-
-
 Cereibacter sphaeroides
F-
-
 Rhodopseudomonas palustris
F-
-
 Rhodopseudomonas rubrum
iodoacetate not Cereibacter sphaeroides
iodoacetate not Rhodopseudomonas palustris
iodoacetate not Rhodopseudomonas rubrum
additional information not inhibitory: p-chloromercuribenzoate Cereibacter sphaeroides
additional information not inhibitory: p-chloromercuribenzoate Rhodopseudomonas palustris
additional information not inhibitory: p-chloromercuribenzoate Rhodopseudomonas rubrum
P1,P5-diadenosine 5'-pentaphosphate kinetics Cereibacter sphaeroides
P1,P5-diadenosine 5'-pentaphosphate kinetics Rhodopseudomonas palustris
P1,P5-diadenosine 5'-pentaphosphate kinetics Rhodopseudomonas rubrum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24 0.27 ADP
-
 Cereibacter sphaeroides
0.24 0.27 ADP
-
 Rhodopseudomonas palustris
0.24 0.27 ADP
-
 Rhodopseudomonas rubrum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Cereibacter sphaeroides 5829
-
cytosol
-
 Rhodopseudomonas palustris 5829
-
cytosol
-
 Rhodopseudomonas rubrum 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ can replace Mg2+, Ca2+ or Mn2+ less efficiently, slight activation Cereibacter sphaeroides
Ba2+ can replace Mg2+, Ca2+ or Mn2+ less efficiently, slight activation Rhodopseudomonas palustris
Ba2+ can replace Mg2+, Ca2+ or Mn2+ less efficiently, slight activation Rhodopseudomonas rubrum
Ca2+ in decreasing order of efficiency: Mg2+, Ca2+, Co2+, Mn2+, Zn2+ Cereibacter sphaeroides
Ca2+ in decreasing order of efficiency: Mg2+, Ca2+, Co2+, Mn2+, Zn2+ Rhodopseudomonas palustris
Ca2+ in decreasing order of efficiency: Mg2+, Ca2+, Co2+, Mn2+, Zn2+ Rhodopseudomonas rubrum
Fe2+ slight activation Cereibacter sphaeroides
Fe2+ slight activation Rhodopseudomonas palustris
Fe2+ slight activation Rhodopseudomonas rubrum
Mg2+ requirement Cereibacter sphaeroides
Mg2+ requirement Rhodopseudomonas palustris
Mg2+ requirement Rhodopseudomonas rubrum
Mg2+ in decreasing order of efficiency: Mg2+, Ca2+, Co2+, Mn2+, Zn2+ Cereibacter sphaeroides
Mg2+ in decreasing order of efficiency: Mg2+, Ca2+, Co2+, Mn2+, Zn2+ Rhodopseudomonas palustris
Mg2+ in decreasing order of efficiency: Mg2+, Ca2+, Co2+, Mn2+, Zn2+ Rhodopseudomonas rubrum
Mg2+ MgADP- is true substrate Cereibacter sphaeroides
Mg2+ MgADP- is true substrate Rhodopseudomonas palustris
Mg2+ MgADP- is true substrate Rhodopseudomonas rubrum
Mn2+ in decreasing order of efficiency: Mg2+, Ca2+, Co2+, Mn2+, Zn2+ Cereibacter sphaeroides
Mn2+ in decreasing order of efficiency: Mg2+, Ca2+, Co2+, Mn2+, Zn2+ Rhodopseudomonas palustris
Mn2+ in decreasing order of efficiency: Mg2+, Ca2+, Co2+, Mn2+, Zn2+ Rhodopseudomonas rubrum
Zn2+ in decreasing order of efficiency: Mg2+, Ca2+, Co2+, Mn2+, Zn2+ Cereibacter sphaeroides
Zn2+ in decreasing order of efficiency: Mg2+, Ca2+, Co2+, Mn2+, Zn2+ Rhodopseudomonas palustris
Zn2+ in decreasing order of efficiency: Mg2+, Ca2+, Co2+, Mn2+, Zn2+ Rhodopseudomonas rubrum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30900
-
 1 * 30900, SDS-PAGE Rhodopseudomonas rubrum
32100
-
 gel filtration Rhodopseudomonas rubrum
32100
-
 1 * 32100, SDS-PAGE Rhodopseudomonas palustris
32800
-
 1 * 32800, SDS-PAGE Cereibacter sphaeroides
33500
-
 gel filtration Rhodopseudomonas palustris
34400
-
 gel filtration Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-
Rhodopseudomonas palustris
-
-
-
Rhodopseudomonas rubrum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cereibacter sphaeroides
-
 Rhodopseudomonas palustris
-
 Rhodopseudomonas rubrum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
76.7 79.3 25°C, pH 8.0 Cereibacter sphaeroides
76.7 79.3 25°C, pH 8.0 Rhodopseudomonas palustris
76.7 79.3 25°C, pH 8.0 Rhodopseudomonas rubrum

Storage Stability

Storage Stability Organism
0°C, at least 7 days Cereibacter sphaeroides
0°C, at least 7 days Rhodopseudomonas palustris
0°C, at least 7 days Rhodopseudomonas rubrum
deep frozen, several weeks Cereibacter sphaeroides
deep frozen, several weeks Rhodopseudomonas palustris
deep frozen, several weeks Rhodopseudomonas rubrum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + ADP
-
 Cereibacter sphaeroides ATP + AMP
-
 r
ADP + ADP
-
 Rhodopseudomonas palustris ATP + AMP
-
 r
ADP + ADP
-
 Rhodopseudomonas rubrum ATP + AMP
-
 r
ATP + AMP
-
 Cereibacter sphaeroides ADP + ADP
-
 r
ATP + AMP
-
 Rhodopseudomonas palustris ADP + ADP
-
 r
ATP + AMP
-
 Rhodopseudomonas rubrum ADP + ADP
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 32800, SDS-PAGE Cereibacter sphaeroides
monomer 1 * 32100, SDS-PAGE Rhodopseudomonas palustris
monomer 1 * 30900, SDS-PAGE Rhodopseudomonas rubrum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Cereibacter sphaeroides
25
-
-
 Rhodopseudomonas palustris
25
-
-
 Rhodopseudomonas rubrum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 t1/2: 10 min Cereibacter sphaeroides
45
-
 t1/2: 10 min Rhodopseudomonas palustris
45
-
 t1/2: 10 min Rhodopseudomonas rubrum
60
-
 t1/2: 1 min Cereibacter sphaeroides
60
-
 t1/2: 1 min Rhodopseudomonas palustris
60
-
 t1/2: 1 min Rhodopseudomonas rubrum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Cereibacter sphaeroides
8
-
-
 Rhodopseudomonas palustris
8
-
-
 Rhodopseudomonas rubrum

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 quite stable for a short time Cereibacter sphaeroides
4
-
 quite stable for a short time Rhodopseudomonas palustris
4
-
 quite stable for a short time Rhodopseudomonas rubrum
12
-
 quite stable for a short time Cereibacter sphaeroides
12
-
 quite stable for a short time Rhodopseudomonas palustris
12
-
 quite stable for a short time Rhodopseudomonas rubrum

pI Value

Organism Comment pI Value Maximum pI Value
Cereibacter sphaeroides isoelectric focusing
-
 4.5
Rhodopseudomonas palustris isoelectric focusing
-
 4.5
Rhodopseudomonas rubrum isoelectric focusing
-
 4.5