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Literature summary for 2.7.4.3 extracted from

  • McKellar, I.R.C.; Charles, A.M.; Butler, B.J.
    Some properties of adenylate kinase from chemolithotropically grown Thiobacillus novellus (1980), Arch. Microbiol., 124, 275-284.
No PubMed abstract available
Search for more literature for 2.7.4.3

Inhibitors

Inhibitors Comment Organism Structure
3-phosphoglyceraldehyde
-
 Starkeya novella
acetyl-CoA
-
 Starkeya novella
AMP product inhibition Starkeya novella
ATP product inhibition Starkeya novella
CoA
-
 Starkeya novella
diphosphate reverse reaction Starkeya novella
Hg2+ strong, not reaction of ADP + ADP Starkeya novella
Mg2+ above 0.5 mM Starkeya novella
additional information effect of varios intermediary metabolites; no inhibition by K+, Na+, NH4+, AsO2, citrate, NADH, fructose 6-phosphate, 2-phosphoglyceraldehyde Starkeya novella
N-ethylmaleimide not Starkeya novella
p-hydroxymercuribenzoate strong, reversible by GSH or cysteine Starkeya novella
phosphoenolpyruvate
-
 Starkeya novella

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.038 0.04 AMP pH 7.5, 25°C, ADP production Starkeya novella
0.09 0.092 ADP3- pH 8.7, 25°C Starkeya novella
0.09 0.092 ADP3- ATP production Starkeya novella
0.34 0.35 ADP pH 8.7, 25°C Starkeya novella

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Starkeya novella 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ about 50% as effective as Mg2+ Starkeya novella
Mg2+ no absolute requirement: 20% of activity in its absence Starkeya novella
Mn2+ requirement, about 25% as effective as Mg2+ Starkeya novella

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 sucrose density gradient centrifugation Starkeya novella
46300
-
 1 * 46300, SDS-PAGE Starkeya novella
47800
-
 1 * 47800, SDS-PAGE after treatment with 5 M urea Starkeya novella

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + ADP Starkeya novella involved in energy metabolism ?
-
 r

Organism

Organism UniProt Comment Textmining
Starkeya novella
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Starkeya novella

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
32
-
 pH 8.7, 25°C, ATP production Starkeya novella

Storage Stability

Storage Stability Organism
-20°C, several weeks Starkeya novella

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + ADP no substrate: CDP Starkeya novella ATP + AMP
-
 r
ADP + ADP no substrates: IDP, GDP Starkeya novella ATP + AMP
-
 r
ADP + ADP involved in energy metabolism Starkeya novella ?
-
 r
ATP + AMP no substrates are adenosine, 2',3'-AMP or 3',5'-AMP Starkeya novella ADP + ADP
-
 r
ATP + AMP no substrate of the reverse reaction: CDP Starkeya novella ADP + ADP
-
 r
ATP + AMP no substrates are ATP/IMP Starkeya novella ADP + ADP
-
 r
ATP + AMP no substrates of the reverse reaction: IDP, GDP Starkeya novella ADP + ADP
-
 r
ATP + AMP specific for ATP, AMP and ADP Starkeya novella ADP + ADP
-
 r
ATP + AMP no substrates are ATP/GMP Starkeya novella ADP + ADP
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 46300, SDS-PAGE Starkeya novella
monomer 1 * 47800, SDS-PAGE after treatment with 5 M urea Starkeya novella

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 5 min, inactivation, 0.5 mM dithiothreitol protects Starkeya novella

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
 AMP + ATP Starkeya novella
8.7
-
 ADP + ADP Starkeya novella