General Stability | Organism |
---|---|
low ionic strength inactivates | Homo sapiens |
Triton X-100, EDTA, dithiothreitol and electrolyte protect enzyme in dilute solution and against denaturation by heat or extreme pH-values | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | product inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.076 | 0.083 | AMP | 25°C, pH 8.0 | Homo sapiens | |
0.09 | 0.092 | ADP3- | 25°C, pH 8.0 | Homo sapiens | |
0.09 | 0.092 | ADP3- | ATP production | Homo sapiens | |
0.11 | - |
ADP | 25°C, pH 8.0 | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Homo sapiens | |
Mg2+ | 1 mM | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21300 | - |
sedimentation equilibrium | Homo sapiens |
21300 | - |
isozyme AKalpha, sedimentation equilibrium | Homo sapiens |
22500 | - |
isozyme AKalpha, gel filtration | Homo sapiens |
23000 | - |
1 * 23000, isozyme AKalpha, SDS-PAGE | Homo sapiens |
23400 | - |
1 * 23400, isozyme AKalpha, sedimentation equilibrium in 6 M guanidine hydrochloride | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
predominant form AKalpha (major form of AK-1 isozymes) | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3200 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
0°C, a few days stable, after which activity drops to 70%, this lower specific activity can be maintained at -20°C in ammonium sulfate solution for a prolonged period | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + ADP | - |
Homo sapiens | ATP + AMP | - |
r | |
ATP + AMP | best substrates | Homo sapiens | ADP + ADP | - |
r | |
ATP + AMP | no substrates are ATP/dGMP | Homo sapiens | ADP + ADP | - |
r | |
ATP + AMP | no substrates are ATP/IMP | Homo sapiens | ADP + ADP | - |
r | |
ATP + AMP | no substrates are dGTP/AMP | Homo sapiens | ADP + ADP | - |
r | |
ATP + AMP | no substrates are ATP/UMP | Homo sapiens | ADP + ADP | - |
r | |
ATP + AMP | no substrates are ATP/GMP | Homo sapiens | ADP + ADP | - |
r | |
ATP + CMP | reaction at 1% the rate of AMP | Homo sapiens | ADP + ? | - |
? | |
ATP + dAMP | reaction at 10% the rate of AMP | Homo sapiens | ADP + dADP | - |
? | |
CTP + AMP | reaction at about 3% the rate of ATP | Homo sapiens | ADP + CDP | - |
? | |
dATP + AMP | reaction at about 50% the rate of ATP | Homo sapiens | dADP + ADP | - |
? | |
ITP + AMP | not ATP/IMP | Homo sapiens | IDP + ADP | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 23400, isozyme AKalpha, sedimentation equilibrium in 6 M guanidine hydrochloride | Homo sapiens |
monomer | 1 * 23000, isozyme AKalpha, SDS-PAGE | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
in 10 mM phosphate buffer, pH 7, 0.1 M KCl, 0.02% Triton X-100, 2 mM DTT, +/- EDTA, t1/2: 10 min, with more than 0.2 M KCl: 10-20% loss of activity within 10 min | Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | 10 min, at 90°C, in 10 mM phosphate buffer, pH 7, 0.1 M KCl, DTT, Triton X-100, +/- EDTA, 10% loss of activity within 10 min | Homo sapiens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Homo sapiens | isozyme AKalpha, isoelectric focusing | - |
9 |