BRENDA - Enzyme Database
show all sequences of 2.7.4.25

The Rv1712 locus from Mycobacterium tuberculosis H37Rv codes for a functional CMP kinase that preferentially phosphorylates dCMP

Thum, C.; Schneider, C.Z.; Palma, M.S.; Santos, D.S.; Basso, L.A.; J. Bacteriol. 191, 2884-2887 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Mycobacterium tuberculosis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.12
-
CMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
0.165
-
dCMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
13.85
-
UMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
21000
-
gel filtration
Mycobacterium tuberculosis
23930
-
ESI-MS
Mycobacterium tuberculosis
24060
-
calculated from amino acid sequence
Mycobacterium tuberculosis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-
Purification (Commentary)
Commentary
Organism
anionic-exchange column chromatography, gel filtration, and anionic-exchange resin column chromatography
Mycobacterium tuberculosis
Storage Stability
Storage Stability
Organism
-80°C, no apparent loss of activity
Mycobacterium tuberculosis
4°C, up to 30 days, recombinant CMK remains stable
Mycobacterium tuberculosis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + CMP
the enzyme preferentially phosphorylates CMP and dCMP
712276
Mycobacterium tuberculosis
ADP + CDP
-
-
-
?
ATP + CMP
the enzyme preferentially phosphorylates CMP and dCMP
712276
Mycobacterium tuberculosis H37Rv
ADP + CDP
-
-
-
?
ATP + dCMP
the enzyme preferentially phosphorylates CMP and dCMP
712276
Mycobacterium tuberculosis
ADP + dCDP
-
-
-
?
ATP + dCMP
the enzyme preferentially phosphorylates CMP and dCMP
712276
Mycobacterium tuberculosis H37Rv
ADP + dCDP
-
-
-
?
ATP + UMP
UMP is a poor substrate
712276
Mycobacterium tuberculosis
ADP + UDP
-
-
-
?
ATP + UMP
UMP is a poor substrate
712276
Mycobacterium tuberculosis H37Rv
ADP + UDP
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 21000, gel filtration
Mycobacterium tuberculosis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
12.2
-
UMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
30
-
dCMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
52
-
CMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Mycobacterium tuberculosis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Mycobacterium tuberculosis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Mycobacterium tuberculosis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.12
-
CMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
0.165
-
dCMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
13.85
-
UMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
21000
-
gel filtration
Mycobacterium tuberculosis
23930
-
ESI-MS
Mycobacterium tuberculosis
24060
-
calculated from amino acid sequence
Mycobacterium tuberculosis
Purification (Commentary) (protein specific)
Commentary
Organism
anionic-exchange column chromatography, gel filtration, and anionic-exchange resin column chromatography
Mycobacterium tuberculosis
Storage Stability (protein specific)
Storage Stability
Organism
-80°C, no apparent loss of activity
Mycobacterium tuberculosis
4°C, up to 30 days, recombinant CMK remains stable
Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + CMP
the enzyme preferentially phosphorylates CMP and dCMP
712276
Mycobacterium tuberculosis
ADP + CDP
-
-
-
?
ATP + CMP
the enzyme preferentially phosphorylates CMP and dCMP
712276
Mycobacterium tuberculosis H37Rv
ADP + CDP
-
-
-
?
ATP + dCMP
the enzyme preferentially phosphorylates CMP and dCMP
712276
Mycobacterium tuberculosis
ADP + dCDP
-
-
-
?
ATP + dCMP
the enzyme preferentially phosphorylates CMP and dCMP
712276
Mycobacterium tuberculosis H37Rv
ADP + dCDP
-
-
-
?
ATP + UMP
UMP is a poor substrate
712276
Mycobacterium tuberculosis
ADP + UDP
-
-
-
?
ATP + UMP
UMP is a poor substrate
712276
Mycobacterium tuberculosis H37Rv
ADP + UDP
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 21000, gel filtration
Mycobacterium tuberculosis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
12.2
-
UMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
30
-
dCMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
52
-
CMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
General Information
General Information
Commentary
Organism
physiological function
the physiological role of CMK is to recycle CMP to CDP, which is either rapidly phosphorylated by the unspecific nucleoside diphosphate kinase NdkA to CTP or reduced to dCDP
Mycobacterium tuberculosis
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the physiological role of CMK is to recycle CMP to CDP, which is either rapidly phosphorylated by the unspecific nucleoside diphosphate kinase NdkA to CTP or reduced to dCDP
Mycobacterium tuberculosis
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.88
-
UMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
200
-
dCMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
1100
-
CMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.88
-
UMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
200
-
dCMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
1100
-
CMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
Other publictions for EC 2.7.4.25
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
712276
Thum
The Rv1712 locus from Mycobact ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Bacteriol.
191
2884-2887
2009
-
-
1
-
-
-
-
3
-
-
3
-
-
160
-
-
1
-
-
-
-
2
6
1
-
-
-
3
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
3
-
-
3
-
-
-
-
1
-
-
-
2
6
1
-
-
-
3
-
-
-
-
-
1
1
-
3
3
693707
Caceres
Molecular modeling and dynamic ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Mol. Model.
14
427-434
2008
-
-
1
-
-
-
-
-
-
1
-
2
-
6
-
-
-
-
-
-
-
-
6
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
2
-
-
-
-
-
-
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1
-
2
-
-
-
-
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673586
Wang
The role of Ureaplasma nucleos ...
Ureaplasma parvum
FEBS J.
274
1983-1990
2007
-
-
1
-
-
-
-
-
-
1
1
-
-
1
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676227
Alexandre
Enantioselectivity of human AM ...
Escherichia coli
Nucleic Acids Res.
35
4895-4904
2007
-
-
1
1
7
-
-
23
-
-
-
-
-
1
-
-
1
-
-
-
-
-
3
-
-
-
6
-
-
-
-
-
-
-
-
-
-
1
-
1
7
-
-
-
-
23
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
692282
Ofiteru
Structural and functional cons ...
Escherichia coli, Escherichia coli K-12
FEBS J.
274
3363-3373
2007
-
-
2
2
14
1
-
27
-
1
1
3
-
2
-
-
1
-
-
-
1
-
8
-
-
-
1
26
-
-
-
2
-
-
-
-
-
2
2
2
14
1
-
-
-
27
-
1
1
3
-
-
-
1
-
-
1
-
8
-
-
-
1
26
-
-
-
-
-
-
-
-
23
23
675215
Lee
-
Recombinant Escherichia coli-c ...
Escherichia coli
J. Ind. Eng. Chem.
12
757-761
2006
-
1
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
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-
-
1
1
-
-
-
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-
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-
1
-
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-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
713473
Yu
Solution structure and functio ...
Streptococcus pneumoniae TIGR4
Protein Sci.
12
2613-2621
2003
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
712736
Bertrand
Sugar specificity of bacterial ...
Escherichia coli
J. Mol. Biol.
315
1099-1110
2002
-
-
1
1
3
-
-
15
-
1
-
2
-
1
-
-
1
-
-
-
-
-
5
-
-
-
1
16
-
-
-
1
-
-
-
-
-
1
1
1
3
-
-
-
-
15
-
1
-
2
-
-
-
1
-
-
-
-
5
-
-
-
1
16
-
-
-
-
-
-
-
-
12
12
645151
Li de La Sierra
Substrate-induced fit of the A ...
Escherichia coli
Biochemistry
39
15870-15878
2000
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645163
Briozzo
Structures of Escherichia coli ...
Escherichia coli
Structure
6
1517-1527
1998
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645159
Schultz
Structural and catalytic prope ...
Bacillus subtilis, Escherichia coli
Arch. Biochem. Biophys.
340
144-153
1997
-
-
1
-
-
-
-
10
-
-
-
-
-
2
-
-
1
-
-
-
-
-
14
-
-
-
6
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
10
-
-
-
-
-
-
-
1
-
-
-
-
14
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
645154
Bucurenci
CMP kinase from Escherichia co ...
Escherichia coli, Escherichia coli K-12
J. Biol. Chem.
271
2856-2862
1996
-
-
1
2
1
-
1
16
-
-
1
-
-
2
-
-
2
-
-
-
-
-
20
1
-
-
1
-
-
-
-
1
-
-
-
-
-
1
1
2
1
-
-
1
-
16
-
-
1
-
-
-
-
2
-
-
-
-
20
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
712269
Fricke
The cmk gene encoding cytidine ...
Escherichia coli K-12
J. Bacteriol.
177
517-523
1995
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
712266
Beck
Salmonella typhimurium mutants ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Bacteriol.
120
1370-1379
1974
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
3
-
-
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-
-
-
-
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-
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