BRENDA - Enzyme Database
show all sequences of 2.7.4.25

Salmonella typhimurium mutants defective in cytidine monophosphate kinase (cmk)

Beck, C.F.; Neuhard, J.; Thomassen, E.; Ingraham, J.L.; Kleker, E.; J. Bacteriol. 120, 1370-1379 (1974)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21(DE3) cells
Salmonella enterica subsp. enterica serovar Typhimurium
Organism
Organism
UniProt
Commentary
Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
strain JL1278
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.0023
-
enzyme from cmk mutant strain KP1300, using CMP as substrate, at pH 8.4 and 37C
Salmonella enterica subsp. enterica serovar Typhimurium
0.0033
-
enzyme from cmk mutant strain JL924, using CMP as substrate, at pH 8.4 and 37C
Salmonella enterica subsp. enterica serovar Typhimurium
0.0177
-
enzyme from cmk wild type strain JL1278, using CMP as substrate, at pH 8.4 and 37C
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ATP + CMP
-
712266
Salmonella enterica subsp. enterica serovar Typhimurium
ADP + CDP
-
-
-
?
ATP + dCMP
-
712266
Salmonella enterica subsp. enterica serovar Typhimurium
ADP + dCDP
-
-
-
?
additional information
CMP kinase from Salmonella typhimurium does not act on UMP
712266
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
-
Synonyms
Synonyms
Commentary
Organism
ATP:CMP phosphotransferase
-
Salmonella enterica subsp. enterica serovar Typhimurium
CMK
-
Salmonella enterica subsp. enterica serovar Typhimurium
CMP kinase
-
Salmonella enterica subsp. enterica serovar Typhimurium
cytidine 5-monophosphate kinase
-
Salmonella enterica subsp. enterica serovar Typhimurium
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Salmonella enterica subsp. enterica serovar Typhimurium
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Salmonella enterica subsp. enterica serovar Typhimurium
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Salmonella enterica subsp. enterica serovar Typhimurium
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.0023
-
enzyme from cmk mutant strain KP1300, using CMP as substrate, at pH 8.4 and 37C
Salmonella enterica subsp. enterica serovar Typhimurium
0.0033
-
enzyme from cmk mutant strain JL924, using CMP as substrate, at pH 8.4 and 37C
Salmonella enterica subsp. enterica serovar Typhimurium
0.0177
-
enzyme from cmk wild type strain JL1278, using CMP as substrate, at pH 8.4 and 37C
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ATP + CMP
-
712266
Salmonella enterica subsp. enterica serovar Typhimurium
ADP + CDP
-
-
-
?
ATP + dCMP
-
712266
Salmonella enterica subsp. enterica serovar Typhimurium
ADP + dCDP
-
-
-
?
additional information
CMP kinase from Salmonella typhimurium does not act on UMP
712266
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
-
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
712276
Thum
The Rv1712 locus from Mycobact ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Bacteriol.
191
2884-2887
2009
-
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1
-
-
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3
-
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3
-
-
172
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1
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2
6
1
2
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3
-
-
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1
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1
1
-
-
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3
-
-
3
-
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-
1
-
-
-
2
6
1
-
-
-
3
-
-
-
-
-
1
1
-
3
3
693707
Caceres
Molecular modeling and dynamic ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Mol. Model.
14
427-434
2008
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-
1
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1
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2
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6
-
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6
1
9
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1
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1
2
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1
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2
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6
1
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-
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-
673586
Wang
The role of Ureaplasma nucleos ...
Ureaplasma parvum
FEBS J.
274
1983-1990
2007
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1
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1
1
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1
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1
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4
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3
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1
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1
1
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-
1
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-
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4
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
676227
Alexandre
Enantioselectivity of human AM ...
Escherichia coli
Nucleic Acids Res.
35
4895-4904
2007
-
-
1
1
7
-
-
23
-
-
-
-
-
1
-
-
1
-
-
-
-
-
3
-
3
-
-
6
-
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-
-
-
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1
-
1
7
-
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23
-
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1
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-
-
-
3
-
-
-
6
-
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-
-
-
-
-
-
-
-
692282
Ofiteru
Structural and functional cons ...
Escherichia coli, Escherichia coli K-12
FEBS J.
274
3363-3373
2007
-
-
2
2
14
1
-
27
-
1
1
3
-
2
-
-
1
-
-
-
1
-
8
-
2
-
-
1
26
-
-
-
2
-
-
-
-
-
2
2
2
14
1
-
-
-
27
-
1
1
3
-
-
-
1
-
-
1
-
8
-
-
-
1
26
-
-
-
-
-
-
-
-
23
23
675215
Lee
-
Recombinant Escherichia coli-c ...
Escherichia coli
J. Ind. Eng. Chem.
12
757-761
2006
-
1
1
-
-
-
-
-
-
1
-
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1
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1
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1
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1
1
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1
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1
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-
713473
Yu
Solution structure and functio ...
Streptococcus pneumoniae TIGR4
Protein Sci.
12
2613-2621
2003
-
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-
-
-
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-
-
-
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-
5
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-
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1
-
2
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1
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1
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1
-
-
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-
-
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1
1
-
-
-
712736
Bertrand
Sugar specificity of bacterial ...
Escherichia coli
J. Mol. Biol.
315
1099-1110
2002
-
-
1
1
3
-
-
15
-
1
-
2
-
1
-
-
1
-
-
-
-
-
5
-
2
-
-
1
16
-
-
-
1
-
-
-
-
-
1
1
1
3
-
-
-
-
15
-
1
-
2
-
-
-
1
-
-
-
-
5
-
-
-
1
16
-
-
-
-
-
-
-
-
12
12
645151
Li de La Sierra
Substrate-induced fit of the A ...
Escherichia coli
Biochemistry
39
15870-15878
2000
-
-
-
-
-
-
-
-
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1
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1
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645163
Briozzo
Structures of Escherichia coli ...
Escherichia coli
Structure
6
1517-1527
1998
-
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1
1
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-
-
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1
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3
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1
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1
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-
-
-
-
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3
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
645159
Schultz
Structural and catalytic prope ...
Bacillus subtilis, Escherichia coli
Arch. Biochem. Biophys.
340
144-153
1997
-
-
1
-
-
-
-
10
-
-
-
-
-
2
-
-
1
-
-
-
-
-
14
-
2
-
-
6
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
10
-
-
-
-
-
-
-
1
-
-
-
-
14
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
645154
Bucurenci
CMP kinase from Escherichia co ...
Escherichia coli, Escherichia coli K-12
J. Biol. Chem.
271
2856-2862
1996
-
-
1
2
1
-
1
16
-
-
1
-
-
2
-
-
2
-
-
-
-
-
20
1
1
-
-
1
-
-
-
-
1
-
-
-
-
-
1
1
2
1
-
-
1
-
16
-
-
1
-
-
-
-
2
-
-
-
-
20
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
712269
Fricke
The cmk gene encoding cytidine ...
Escherichia coli K-12
J. Bacteriol.
177
517-523
1995
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
3
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
712266
Beck
Salmonella typhimurium mutants ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Bacteriol.
120
1370-1379
1974
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
3
-
4
-
-
-
-
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-
-
1
-
-
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-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
3
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