BRENDA - Enzyme Database
show all sequences of 2.7.4.25

Structures of Escherichia coli CMP kinase alone and in complex with CDP: a new fold of the nucleoside monophosphate binding domain and insights into cytosine nucleotide specificity

Briozzo, P.; Golinelli-Pimpaneau, B.; Gilles, A.M.; Gaucher, J.F.; Burlacu-Miron, S.; Sakamoto, H.; Janin, J.; Barzu, O.; Structure 6, 1517-1527 (1998)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
hanging-drop method in 50 mM Tris-HCl buffer, pH 7.4, 20°C, with ammonium sulfate as a precipitant
Escherichia coli
Crystallization (Commentary)
Crystallization (Commentary)
Organism
enzyme in complex with CDP
Escherichia coli
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ATP + CMP
-
645163
Escherichia coli
ADP + CDP
-
-
-
?
ATP + dCMP
105% of the activity with CMP
645163
Escherichia coli
ADP + dCDP
-
-
-
?
ATP + UMP
0.8% of the activity with CMP
645163
Escherichia coli
ADP + UDP
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
hanging-drop method in 50 mM Tris-HCl buffer, pH 7.4, 20°C, with ammonium sulfate as a precipitant
Escherichia coli
Crystallization (Commentary) (protein specific)
Crystallization
Organism
enzyme in complex with CDP
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ATP + CMP
-
645163
Escherichia coli
ADP + CDP
-
-
-
?
ATP + dCMP
105% of the activity with CMP
645163
Escherichia coli
ADP + dCDP
-
-
-
?
ATP + UMP
0.8% of the activity with CMP
645163
Escherichia coli
ADP + UDP
-
-
-
?
Other publictions for EC 2.7.4.25
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
712276
Thum
The Rv1712 locus from Mycobact ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Bacteriol.
191
2884-2887
2009
-
-
1
-
-
-
-
3
-
-
3
-
-
172
-
-
1
-
-
-
-
2
6
1
2
-
-
-
3
-
-
-
1
-
-
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1
1
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3
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3
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1
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2
6
1
-
-
-
3
-
-
-
-
-
1
1
-
3
3
693707
Caceres
Molecular modeling and dynamic ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Mol. Model.
14
427-434
2008
-
-
1
-
-
-
-
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-
1
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2
-
6
-
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6
1
9
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1
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1
2
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6
1
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673586
Wang
The role of Ureaplasma nucleos ...
Ureaplasma parvum
FEBS J.
274
1983-1990
2007
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1
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1
1
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1
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1
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1
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4
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676227
Alexandre
Enantioselectivity of human AM ...
Escherichia coli
Nucleic Acids Res.
35
4895-4904
2007
-
-
1
1
7
-
-
23
-
-
-
-
-
1
-
-
1
-
-
-
-
-
3
-
3
-
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6
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1
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1
7
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23
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1
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-
3
-
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6
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-
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-
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-
-
-
-
692282
Ofiteru
Structural and functional cons ...
Escherichia coli, Escherichia coli K-12
FEBS J.
274
3363-3373
2007
-
-
2
2
14
1
-
27
-
1
1
3
-
2
-
-
1
-
-
-
1
-
8
-
2
-
-
1
26
-
-
-
2
-
-
-
-
-
2
2
2
14
1
-
-
-
27
-
1
1
3
-
-
-
1
-
-
1
-
8
-
-
-
1
26
-
-
-
-
-
-
-
-
23
23
675215
Lee
-
Recombinant Escherichia coli-c ...
Escherichia coli
J. Ind. Eng. Chem.
12
757-761
2006
-
1
1
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1
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1
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1
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1
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1
1
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1
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713473
Yu
Solution structure and functio ...
Streptococcus pneumoniae TIGR4
Protein Sci.
12
2613-2621
2003
-
-
-
-
-
-
-
-
-
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5
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1
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2
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1
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1
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1
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1
1
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712736
Bertrand
Sugar specificity of bacterial ...
Escherichia coli
J. Mol. Biol.
315
1099-1110
2002
-
-
1
1
3
-
-
15
-
1
-
2
-
1
-
-
1
-
-
-
-
-
5
-
2
-
-
1
16
-
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1
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1
1
1
3
-
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15
-
1
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2
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1
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5
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1
16
-
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12
12
645151
Li de La Sierra
Substrate-induced fit of the A ...
Escherichia coli
Biochemistry
39
15870-15878
2000
-
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1
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1
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-
-
-
-
-
645163
Briozzo
Structures of Escherichia coli ...
Escherichia coli
Structure
6
1517-1527
1998
-
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1
1
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1
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3
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1
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1
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3
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645159
Schultz
Structural and catalytic prope ...
Bacillus subtilis, Escherichia coli
Arch. Biochem. Biophys.
340
144-153
1997
-
-
1
-
-
-
-
10
-
-
-
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2
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1
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14
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2
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6
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1
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1
1
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10
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14
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6
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-
-
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-
645154
Bucurenci
CMP kinase from Escherichia co ...
Escherichia coli, Escherichia coli K-12
J. Biol. Chem.
271
2856-2862
1996
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1
2
1
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1
16
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1
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2
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20
1
1
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1
2
1
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1
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16
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1
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20
1
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1
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-
712269
Fricke
The cmk gene encoding cytidine ...
Escherichia coli K-12
J. Bacteriol.
177
517-523
1995
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3
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2
2
-
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-
712266
Beck
Salmonella typhimurium mutants ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Bacteriol.
120
1370-1379
1974
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1
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