BRENDA - Enzyme Database
show all sequences of 2.7.4.25

Structural and catalytic properties of CMP kinase from Bacillus subtilis: a comparative analysis with the homologous enzyme from Escherichia coli

Schultz, C.P.; Ylisastigui-Pons, L.; Serina, L.; Sakamoto, H.; Mantsch, H.H.; Neuhard, J.; Barzu, O.; Gilles, A.M.; Arch. Biochem. Biophys. 340, 144-153 (1997)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Bacillus subtilis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.035
-
CMP
pH 7.4, 30°C
Escherichia coli
0.04
-
CMP
at 30°C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH; pH 7.4, 30°C
Bacillus subtilis
0.094
-
dCMP
pH 7.4, 30°C
Escherichia coli
0.12
-
ATP
at 30°C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH
Bacillus subtilis
0.33
-
dCMP
at 30°C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH; pH 7.4, 30°C
Bacillus subtilis
0.36
-
araCMP
pH 7.4, 30°C
Escherichia coli
0.37
-
ara-CMP
at 30°C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH
Bacillus subtilis
0.37
-
araCMP
pH 7.4, 30°C
Bacillus subtilis
0.93
-
UMP
pH 7.4, 30°C
Escherichia coli
3.6
-
UMP
at 30°C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH; pH 7.4, 30°C
Bacillus subtilis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus subtilis
-
-
-
Escherichia coli
-
-
-
Purification (Commentary)
Commentary
Organism
-
Bacillus subtilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + ara-CMP
-
645159
Escherichia coli
ADP + ara-CDP
-
-
-
-
ATP + ara-CMP
ara-CMP is phosphorylated at rate which represents 14% of that with CMP
645159
Bacillus subtilis
ADP + ara-CDP
-
-
-
?
ATP + CMP
-
645159
Bacillus subtilis
ADP + CDP
-
-
-
?
ATP + CMP
-
645159
Escherichia coli
ADP + CDP
-
-
-
?
ATP + CMP
CMP and dCMP are the best phosphate acceptors
645159
Bacillus subtilis
ADP + CDP
-
-
-
?
ATP + dCMP
-
645159
Escherichia coli
ADP + dCDP
-
-
-
-
ATP + dCMP
CMP and dCMP are the best phosphate acceptors
645159
Bacillus subtilis
ADP + dCDP
-
-
-
?
ATP + GTP
GTP is a poor substrate with Bacillus subtilis CMP kinase
645159
Bacillus subtilis
ADP + GDP
-
-
-
?
ATP + UMP
-
645159
Bacillus subtilis
ADP + UDP
-
-
-
?
ATP + UMP
-
645159
Escherichia coli
ADP + UDP
-
-
-
?
ATP + UMP
UMP is phosphorylated at rate which represents 10% of that with CMP
645159
Bacillus subtilis
ADP + UDP
-
-
-
?
dATP + CMP
-
645159
Escherichia coli
dADP + CDP
-
-
-
?
GTP + CMP
poor substrate
645159
Escherichia coli
GDP + CDP
-
-
-
?
GTP + CMP
ATP is equally effective as ATP
645159
Bacillus subtilis
GDP + CDP
-
-
-
-
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
48
-
midpoint denaturation temperature in absence of nucleotide substrates or in presence of ATP
Escherichia coli
48.5
57.8
the melting temperature of CMP kinae in the absence of nucleotide substrates is at 48.5°C, the melting temperature of CMP kinae in the presence of ATP is at 57.8°C, the melting temperature of CMP kinae in the presence of CMP is at 50.5°C
Bacillus subtilis
49
-
midpoint denaturation temperature in absence of nucleotide substrates
Bacillus subtilis
49
-
midpoint denaturation temperature in presence of CMP
Escherichia coli
51
-
midpoint denaturation temperature in presence of CMP
Bacillus subtilis
58
-
midpoint denaturation temperature in presence of ATP
Bacillus subtilis
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Bacillus subtilis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Bacillus subtilis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Bacillus subtilis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.035
-
CMP
pH 7.4, 30°C
Escherichia coli
0.04
-
CMP
at 30°C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH; pH 7.4, 30°C
Bacillus subtilis
0.094
-
dCMP
pH 7.4, 30°C
Escherichia coli
0.12
-
ATP
at 30°C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH
Bacillus subtilis
0.33
-
dCMP
at 30°C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH; pH 7.4, 30°C
Bacillus subtilis
0.36
-
araCMP
pH 7.4, 30°C
Escherichia coli
0.37
-
ara-CMP
at 30°C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH
Bacillus subtilis
0.37
-
araCMP
pH 7.4, 30°C
Bacillus subtilis
0.93
-
UMP
pH 7.4, 30°C
Escherichia coli
3.6
-
UMP
at 30°C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH; pH 7.4, 30°C
Bacillus subtilis
Purification (Commentary) (protein specific)
Commentary
Organism
-
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + ara-CMP
-
645159
Escherichia coli
ADP + ara-CDP
-
-
-
-
ATP + ara-CMP
ara-CMP is phosphorylated at rate which represents 14% of that with CMP
645159
Bacillus subtilis
ADP + ara-CDP
-
-
-
?
ATP + CMP
-
645159
Bacillus subtilis
ADP + CDP
-
-
-
?
ATP + CMP
-
645159
Escherichia coli
ADP + CDP
-
-
-
?
ATP + CMP
CMP and dCMP are the best phosphate acceptors
645159
Bacillus subtilis
ADP + CDP
-
-
-
?
ATP + dCMP
-
645159
Escherichia coli
ADP + dCDP
-
-
-
-
ATP + dCMP
CMP and dCMP are the best phosphate acceptors
645159
Bacillus subtilis
ADP + dCDP
-
-
-
?
ATP + GTP
GTP is a poor substrate with Bacillus subtilis CMP kinase
645159
Bacillus subtilis
ADP + GDP
-
-
-
?
ATP + UMP
-
645159
Bacillus subtilis
ADP + UDP
-
-
-
?
ATP + UMP
-
645159
Escherichia coli
ADP + UDP
-
-
-
?
ATP + UMP
UMP is phosphorylated at rate which represents 10% of that with CMP
645159
Bacillus subtilis
ADP + UDP
-
-
-
?
dATP + CMP
-
645159
Escherichia coli
dADP + CDP
-
-
-
?
GTP + CMP
poor substrate
645159
Escherichia coli
GDP + CDP
-
-
-
?
GTP + CMP
ATP is equally effective as ATP
645159
Bacillus subtilis
GDP + CDP
-
-
-
-
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
48
-
midpoint denaturation temperature in absence of nucleotide substrates or in presence of ATP
Escherichia coli
48.5
57.8
the melting temperature of CMP kinae in the absence of nucleotide substrates is at 48.5°C, the melting temperature of CMP kinae in the presence of ATP is at 57.8°C, the melting temperature of CMP kinae in the presence of CMP is at 50.5°C
Bacillus subtilis
49
-
midpoint denaturation temperature in absence of nucleotide substrates
Bacillus subtilis
49
-
midpoint denaturation temperature in presence of CMP
Escherichia coli
51
-
midpoint denaturation temperature in presence of CMP
Bacillus subtilis
58
-
midpoint denaturation temperature in presence of ATP
Bacillus subtilis
Other publictions for EC 2.7.4.25
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
712276
Thum
The Rv1712 locus from Mycobact ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Bacteriol.
191
2884-2887
2009
-
-
1
-
-
-
-
3
-
-
3
-
-
160
-
-
1
-
-
-
-
2
6
1
-
-
-
3
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
3
-
-
3
-
-
-
-
1
-
-
-
2
6
1
-
-
-
3
-
-
-
-
-
1
1
-
3
3
693707
Caceres
Molecular modeling and dynamic ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Mol. Model.
14
427-434
2008
-
-
1
-
-
-
-
-
-
1
-
2
-
6
-
-
-
-
-
-
-
-
6
1
-
-
-
-
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-
1
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1
2
-
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1
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2
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-
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-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673586
Wang
The role of Ureaplasma nucleos ...
Ureaplasma parvum
FEBS J.
274
1983-1990
2007
-
-
1
-
-
-
-
-
-
1
1
-
-
1
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676227
Alexandre
Enantioselectivity of human AM ...
Escherichia coli
Nucleic Acids Res.
35
4895-4904
2007
-
-
1
1
7
-
-
23
-
-
-
-
-
1
-
-
1
-
-
-
-
-
3
-
-
-
6
-
-
-
-
-
-
-
-
-
-
1
-
1
7
-
-
-
-
23
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
692282
Ofiteru
Structural and functional cons ...
Escherichia coli, Escherichia coli K-12
FEBS J.
274
3363-3373
2007
-
-
2
2
14
1
-
27
-
1
1
3
-
2
-
-
1
-
-
-
1
-
8
-
-
-
1
26
-
-
-
2
-
-
-
-
-
2
2
2
14
1
-
-
-
27
-
1
1
3
-
-
-
1
-
-
1
-
8
-
-
-
1
26
-
-
-
-
-
-
-
-
23
23
675215
Lee
-
Recombinant Escherichia coli-c ...
Escherichia coli
J. Ind. Eng. Chem.
12
757-761
2006
-
1
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
713473
Yu
Solution structure and functio ...
Streptococcus pneumoniae TIGR4
Protein Sci.
12
2613-2621
2003
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
712736
Bertrand
Sugar specificity of bacterial ...
Escherichia coli
J. Mol. Biol.
315
1099-1110
2002
-
-
1
1
3
-
-
15
-
1
-
2
-
1
-
-
1
-
-
-
-
-
5
-
-
-
1
16
-
-
-
1
-
-
-
-
-
1
1
1
3
-
-
-
-
15
-
1
-
2
-
-
-
1
-
-
-
-
5
-
-
-
1
16
-
-
-
-
-
-
-
-
12
12
645151
Li de La Sierra
Substrate-induced fit of the A ...
Escherichia coli
Biochemistry
39
15870-15878
2000
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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-
1
-
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-
-
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-
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-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645163
Briozzo
Structures of Escherichia coli ...
Escherichia coli
Structure
6
1517-1527
1998
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
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1
-
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645159
Schultz
Structural and catalytic prope ...
Bacillus subtilis, Escherichia coli
Arch. Biochem. Biophys.
340
144-153
1997
-
-
1
-
-
-
-
10
-
-
-
-
-
2
-
-
1
-
-
-
-
-
14
-
-
-
6
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
10
-
-
-
-
-
-
-
1
-
-
-
-
14
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
645154
Bucurenci
CMP kinase from Escherichia co ...
Escherichia coli, Escherichia coli K-12
J. Biol. Chem.
271
2856-2862
1996
-
-
1
2
1
-
1
16
-
-
1
-
-
2
-
-
2
-
-
-
-
-
20
1
-
-
1
-
-
-
-
1
-
-
-
-
-
1
1
2
1
-
-
1
-
16
-
-
1
-
-
-
-
2
-
-
-
-
20
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
712269
Fricke
The cmk gene encoding cytidine ...
Escherichia coli K-12
J. Bacteriol.
177
517-523
1995
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
712266
Beck
Salmonella typhimurium mutants ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Bacteriol.
120
1370-1379
1974
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-