Literature summary for 2.7.4.22 extracted from

Hari Prasad, O.; Nanda Kumar, Y.; Reddy, O.V.; Chaudhary, A.; Sarma, P.V.
Cloning, expression, purification and characterization of UMP kinase from Staphylococcus aureus (2012), Protein J., 31, 345-352.

Search for more literature for 2.7.4.22

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli DH5alpha cells	Staphylococcus aureus

Inhibitors

Inhibitors	Comment	Organism	Structure
GTP	when 0.5 M GTP is added in the reaction mixture the affinity of the enzyme towards its UMP substrate decreases almost by 3folds. GTP increases the affinity of ATP towards the enzyme	Staphylococcus aureus
UTP	potent competitive inhibitor	Staphylococcus aureus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00276	-	UMP	purified enzyme, in 50 mM Tris-HCl pH 7.4, at 30°C	Staphylococcus aureus
0.00281	-	UMP	crude cytosolic extract, in 50 mM Tris-HCl pH 7.4, at 30°C	Staphylococcus aureus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Staphylococcus aureus	5829	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
25000	-	6 * 25000, SDS-PAGE	Staphylococcus aureus
150000	-	gel filtration	Staphylococcus aureus

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	B8XPY6	-	-
Staphylococcus aureus ATCC 12600	B8XPY6	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, DEAE cellulose column chromatography, Sephadex G-200 gel filtration, and nickel metal chelate affinity chromatography	Staphylococcus aureus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + UMP	-	Staphylococcus aureus	ADP + UDP	-	?
ATP + UMP	-	Staphylococcus aureus ATCC 12600	ADP + UDP	-	?

Subunits

Subunits	Comment	Organism
homohexamer	6 * 25000, SDS-PAGE	Staphylococcus aureus

Synonyms

Synonyms	Comment	Organism
UMPK	-	Staphylococcus aureus
uridine monophosphate kinase	-	Staphylococcus aureus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0009	-	UTP	in 50 mM Tris-HCl pH 7.4, at 30°C	Staphylococcus aureus

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Release 2023.1 (January 2023)