KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | detailed kinetics, Bi Bi sequential ordered mechanism with phosphomevalonate as the first substrate and ADP the last product, the enzyme follows hyperbolic kinetics and a sequential mechanism, initial velocity and isotope exchange at equilibrium measurements, overview | Sus scrofa | |
0.012 | - |
(R)-5-phosphomevalonate | pH 7.5, 30°C | Sus scrofa | |
0.043 | - |
ATP | pH 7.5, 30°C | Sus scrofa | |
0.043 | - |
ATP | ATP in form of MgATP2- | Sus scrofa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the enzyme requires cations with Mg2+ being the most effective | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-5-phosphomevalonate | Sus scrofa | one of the initial steps in the biosynthesis of steroids and isoprenoids | ADP + (R)-5-diphosphomevalonate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from liver | Sus scrofa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + (R)-5-phosphomevalonate = ADP + (R)-5-diphosphomevalonate | kinetic mechanism, the enzyme follows hyperbolic kinetics and a sequential mechanism | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.16 | - |
purified enzyme | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-5-phosphomevalonate | - |
Sus scrofa | ADP + (R)-5-diphosphomevalonate | - |
r | |
ATP + (R)-5-phosphomevalonate | one of the initial steps in the biosynthesis of steroids and isoprenoids | Sus scrofa | ADP + (R)-5-diphosphomevalonate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 21000-22500, SDS-PAGE | Sus scrofa |
Synonyms | Comment | Organism |
---|---|---|
phosphomevalonate kinase | - |
Sus scrofa |
PMK | - |
Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | - |
Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Sus scrofa | |
ATP | - |
Sus scrofa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics, product inhibition patterns | Sus scrofa |