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Literature summary for 2.7.4.2 extracted from

  • Romanowski, M.J.; Bonanno, J.B.; Burley, S.K.
    Crystal structure of the Streptococcus pneumoniae phosphomevalonate kinase, a member of the GHMP kinase superfamily (2002), Proteins Struct. Funct. Genet., 47, 568-571.
    View publication on PubMed
Search for more literature for 2.7.4.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Staphylococcus aureus
expression in Escherichia coli Streptococcus pneumoniae

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapor diffusion at 20°C against a reservoir containing 100 mM HEPES pH 7.5, 800 mM NaH2PO4, 8000 mM KH2PO4 and 30 mM unbuffered ATP, crystals diffract to 2.4 A resolution Streptococcus pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37747
-
 x * 37747, MALDI mass spectroscopy Streptococcus pneumoniae

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-
Streptococcus pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Se-Met fusion protein, glutathione and Sepharose Q resins Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-phosphomevalonate
-
 Staphylococcus aureus ADP + 5-diphosphomevalonate
-
 ?
ATP + 5-phosphomevalonate
-
 Streptococcus pneumoniae ADP + 5-diphosphomevalonate
-
 ?

Subunits

Subunits Comment Organism
? x * 37747, MALDI mass spectroscopy Streptococcus pneumoniae
More monomeric in solution, hexameric in crystals Streptococcus pneumoniae