General Stability | Organism |
---|---|
sulfhydryl reagents are not essential to protect the activity of phosphomevalonate kinase | Catharanthus roseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
ATP | pH 7.5, 30°C | Catharanthus roseus | |
0.35 | - |
phosphomevalonate | pH 7.5, 30°C | Catharanthus roseus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | divalent cation required for activity, Mg2+ most effective | Catharanthus roseus | |
Mn2+ | 52% of activity with Mg2+ | Catharanthus roseus | |
Ni2+ | 4.7% of activity with Mg2+ | Catharanthus roseus | |
Zn2+ | 22% of activity with Mg2+ | Catharanthus roseus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
128000 | - |
gel filtration | Catharanthus roseus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-5-phosphomevalonate | Catharanthus roseus | involved in isoprenoid biosynthesis pathway | ADP + 5-diphosphomevalonate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Catharanthus roseus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q Sepharose, Phenyl Sepharose, Sephacryl S200, Mono Q, gel filtration, Shodex KW803 | Catharanthus roseus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Catharanthus roseus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.21 | - |
- |
Catharanthus roseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-5-phosphomevalonate | involved in isoprenoid biosynthesis pathway | Catharanthus roseus | ADP + 5-diphosphomevalonate | - |
? | |
ATP + 5-phosphomevalonate | - |
Catharanthus roseus | ADP + 5-diphosphomevalonate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | sharp drop in activity below pH 7.0 and above pH 9.0 | Catharanthus roseus |