Cloned (Comment) | Organism |
---|---|
cDNA is subcloned into pGEX-4T-3 and expressed as a glutathione S-transferase fusion protein in Escherichia coli | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
P1,P5-di(adenosine-5')pentaphosphate | - |
Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
ATP | pH 6.5, when UMP is the other substrate | Arabidopsis thaliana | |
0.153 | - |
UMP | pH 6.5 | Arabidopsis thaliana | |
0.266 | - |
CMP | pH 6.5 | Arabidopsis thaliana | |
0.292 | - |
ATP | pH 6.5, reaction with CMP | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22448 | - |
x * 22448, mass spectroscopy, calculation from nucleotide sequence | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O04905 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + UMP = ADP + UDP | random bi-bi mechanism | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + CMP | - |
Arabidopsis thaliana | ADP + CDP | - |
? | |
ATP + UMP | - |
Arabidopsis thaliana | ADP + UDP | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 22448, mass spectroscopy, calculation from nucleotide sequence | Arabidopsis thaliana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
58 | - |
10 min, 50% loss of activity | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.64 | - |
UMP | pH 6.5 | Arabidopsis thaliana | |
8.57 | - |
CMP | pH 6.5 | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
P1,P5-di(adenosine-5') pentaphosphate | versus ATP | Arabidopsis thaliana | |
0.00653 | - |
P1,P5-di(adenosine-5') pentaphosphate | versus UMP | Arabidopsis thaliana |