Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Tequintavirus T5 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28670 | - |
calculated from nucleotid sequence | Tequintavirus T5 |
29140 | - |
SDS-PAGE | Tequintavirus T5 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deoxynucleoside monophosphate | Tequintavirus T5 | key step in de novo DNA precursor biosynthesis | ADP + deoxynucleoside diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequintavirus T5 | Q6QGP4 | - |
- |
Purification (Comment) | Organism |
---|---|
two alternative methods, the first involves ion-exchange chromatography on Mono Q, the second involves affinity chromatography on Toyopearl AF-Red, the protein is purified 5.34fold in both cases | Tequintavirus T5 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
29 | - |
recombinant protein in sonicated cell exctracts, with dGMP as substrate | Tequintavirus T5 |
36 | - |
recombinant protein after Toyopearl 650 M flow-through, with dGMP as substrate | Tequintavirus T5 |
40 | - |
recombinant protein after ammonium sulphate precipitation, with dGMP as substrate | Tequintavirus T5 |
84.2 | - |
homogeneous recombinant enzyme with dCMP as substrate | Tequintavirus T5 |
153.6 | - |
homogeneous recombinant enzyme with dGMP as substrate | Tequintavirus T5 |
155 | - |
affinity chromatography eluant of the recombinant protein, with dGMP as substrate | Tequintavirus T5 |
155 | - |
ion-exchange chromatography MonoQ eluant of the recombinant protein, with dGMP as substrate | Tequintavirus T5 |
166.8 | - |
homogeneous recombinant enzyme with dTMP as substrate | Tequintavirus T5 |
232 | - |
homogeneous recombinant enzyme with dAMP as substrate | Tequintavirus T5 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dAMP | - |
Tequintavirus T5 | ADP + dADP | - |
? | |
ATP + dCMP | - |
Tequintavirus T5 | ADP + dCDP | - |
? | |
ATP + deoxynucleoside monophosphate | key step in de novo DNA precursor biosynthesis | Tequintavirus T5 | ADP + deoxynucleoside diphosphate | - |
? | |
ATP + dGMP | - |
Tequintavirus T5 | ADP + dGDP | - |
? | |
ATP + dTMP | - |
Tequintavirus T5 | ADP + dTDP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
deoxyribonucleoside monophosphate kinase | - |
Tequintavirus T5 |
dNMP kinase | - |
Tequintavirus T5 |