BRENDA - Enzyme Database
show all sequences of 2.7.4.13

The enzymology of virus-infected bacteria. VI. Purification and properties of the deoxynucleotide kinase induced by bacteriophage T5

Bessman, M.J.; Herriott, S.T.; Van Bibber Orr, M.J.; J. Biol. Chem. 240, 439-445 (1965)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
dAMP
competitive, dTMP as substrate
Escherichia virus T5
dCMP
competitive, dTMP as substrate
Escherichia virus T5
dGMP
competitive, dTMP as substrate
Escherichia virus T5
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.034
-
dCMP
pH 7.5, 37°C
Escherichia virus T5
0.173
-
dTMP
pH 7.5, 37°C
Escherichia virus T5
0.22
-
dAMP
pH 7.5, 37°C
Escherichia virus T5
0.22
-
dGMP
pH 7.5, 37°C
Escherichia virus T5
0.36
-
ATP
pH 7.5, 37°C
Escherichia virus T5
2.8
-
dUMP
pH 7.5, 37°C
Escherichia virus T5
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
requirement, can replace Mg2+
Escherichia virus T5
Fe2+
activation, less effective than Mg2+, Co2+, Mn2+
Escherichia virus T5
Mg2+
requirement, can replace Co2+
Escherichia virus T5
Mn2+
requirement, can replace Co2+ or Mg2+
Escherichia virus T5
additional information
no activation by Ba2+, Ca2+, Cd2+, Cr3+, Cu2+, Fe3+, Hg2+, Ni2+, Zn2+
Escherichia virus T5
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + deoxynucleoside phosphate
Escherichia virus T5
enzyme induced during viral infection
ADP + deoxynucleoside diphosphate
-
Escherichia virus T5
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia virus T5
-
from infected Escherichia coli cells
-
no activity in Escherichia coli
-
-
-
Purification (Commentary)
Commentary
Organism
500fold
Escherichia virus T5
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
14.7
-
purified enzyme
Escherichia virus T5
Storage Stability
Storage Stability
Organism
0°C, 25% loss of activity per day in crude cell extract
Escherichia virus T5
4°C, several months, ionic strength 0.1 or above
Escherichia virus T5
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 5-bromo-dUMP
phosphorylated at 45% the rate of dAMP
642542
Escherichia virus T5
ADP + 5-bromo-dUDP
-
-
-
r
ATP + dAMP
dAMP is the best acceptor substrate
642542
Escherichia virus T5
ADP + dADP
-
642542
Escherichia virus T5
r
ATP + dAMP
dATP can replace ATP with less efficiency
642542
Escherichia virus T5
ADP + dADP
-
642542
Escherichia virus T5
r
ATP + dCMP
phosphorylated at 30% the rate of dAMP, dATP can replace ATP
642542
Escherichia virus T5
ADP + dCDP
-
642542
Escherichia virus T5
r
ATP + deoxynucleoside phosphate
enzyme induced during viral infection
642542
Escherichia virus T5
ADP + deoxynucleoside diphosphate
-
642542
Escherichia virus T5
r
ATP + dGMP
dATP can replace ATP
642542
Escherichia virus T5
ADP + dGDP
-
642542
Escherichia virus T5
r
ATP + dGMP
phosphorylated at 65% the rate of dAMP
642542
Escherichia virus T5
ADP + dGDP
-
642542
Escherichia virus T5
r
ATP + dTMP
dATP can replace ATP with less efficiency
642542
Escherichia virus T5
ADP + dTDP
-
642542
Escherichia virus T5
r
ATP + dTMP
phosphorylated at 70% the rate of dAMP
642542
Escherichia virus T5
ADP + dTDP
-
642542
Escherichia virus T5
r
additional information
very poor acceptor substrates are AMP, CMP or dUMP
642542
Escherichia virus T5
?
-
-
-
-
additional information
no acceptor substrates are dIMP, deoxyxanthylate, 5-hydroxymethyldeoxycytidylate, UMP, GMP, 5-bromo-dUMP, 5-methyl-dCMP or deoxythymidine
642542
Escherichia virus T5
?
-
-
-
-
additional information
no donor substrates are UTP, GTP, CTP, dTTP, dGTP or dCTP
642542
Escherichia virus T5
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Escherichia virus T5
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Escherichia virus T5
7.6
-
substrate dTMP
Escherichia virus T5
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.2
9
about 80% of maximal activity at pH 6.2 and about 65% of maximal activity at pH 9 with substrate dGMP
Escherichia virus T5
6.2
9.4
about 65% of maximal activity at pH 6.2 and about 70% of maximal activity at pH 9.4 with substrate dAMP
Escherichia virus T5
6.3
9
at pH 6.3: about 70% of maximal activity with substrate dTMP, about 85% of maximal activity with substrate dCMP, at pH 9.0: about 50% of maximal activity with substrate dTMP, 65% of maximal activity with substrate dCMP
Escherichia virus T5
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.032
-
dCMP
dTMP as substrate, pH 7.6, 37°C
Escherichia virus T5
0.15
-
dAMP
dTMP as substrate, pH 7.6, 37°C
Escherichia virus T5
0.15
-
dGMP
dTMP as substrate, pH 7.6, 37°C
Escherichia virus T5
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
dAMP
competitive, dTMP as substrate
Escherichia virus T5
dCMP
competitive, dTMP as substrate
Escherichia virus T5
dGMP
competitive, dTMP as substrate
Escherichia virus T5
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.032
-
dCMP
dTMP as substrate, pH 7.6, 37°C
Escherichia virus T5
0.15
-
dAMP
dTMP as substrate, pH 7.6, 37°C
Escherichia virus T5
0.15
-
dGMP
dTMP as substrate, pH 7.6, 37°C
Escherichia virus T5
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.034
-
dCMP
pH 7.5, 37°C
Escherichia virus T5
0.173
-
dTMP
pH 7.5, 37°C
Escherichia virus T5
0.22
-
dAMP
pH 7.5, 37°C
Escherichia virus T5
0.22
-
dGMP
pH 7.5, 37°C
Escherichia virus T5
0.36
-
ATP
pH 7.5, 37°C
Escherichia virus T5
2.8
-
dUMP
pH 7.5, 37°C
Escherichia virus T5
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
requirement, can replace Mg2+
Escherichia virus T5
Fe2+
activation, less effective than Mg2+, Co2+, Mn2+
Escherichia virus T5
Mg2+
requirement, can replace Co2+
Escherichia virus T5
Mn2+
requirement, can replace Co2+ or Mg2+
Escherichia virus T5
additional information
no activation by Ba2+, Ca2+, Cd2+, Cr3+, Cu2+, Fe3+, Hg2+, Ni2+, Zn2+
Escherichia virus T5
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + deoxynucleoside phosphate
Escherichia virus T5
enzyme induced during viral infection
ADP + deoxynucleoside diphosphate
-
Escherichia virus T5
r
Purification (Commentary) (protein specific)
Commentary
Organism
500fold
Escherichia virus T5
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
14.7
-
purified enzyme
Escherichia virus T5
Storage Stability (protein specific)
Storage Stability
Organism
0°C, 25% loss of activity per day in crude cell extract
Escherichia virus T5
4°C, several months, ionic strength 0.1 or above
Escherichia virus T5
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 5-bromo-dUMP
phosphorylated at 45% the rate of dAMP
642542
Escherichia virus T5
ADP + 5-bromo-dUDP
-
-
-
r
ATP + dAMP
dAMP is the best acceptor substrate
642542
Escherichia virus T5
ADP + dADP
-
642542
Escherichia virus T5
r
ATP + dAMP
dATP can replace ATP with less efficiency
642542
Escherichia virus T5
ADP + dADP
-
642542
Escherichia virus T5
r
ATP + dCMP
phosphorylated at 30% the rate of dAMP, dATP can replace ATP
642542
Escherichia virus T5
ADP + dCDP
-
642542
Escherichia virus T5
r
ATP + deoxynucleoside phosphate
enzyme induced during viral infection
642542
Escherichia virus T5
ADP + deoxynucleoside diphosphate
-
642542
Escherichia virus T5
r
ATP + dGMP
dATP can replace ATP
642542
Escherichia virus T5
ADP + dGDP
-
642542
Escherichia virus T5
r
ATP + dGMP
phosphorylated at 65% the rate of dAMP
642542
Escherichia virus T5
ADP + dGDP
-
642542
Escherichia virus T5
r
ATP + dTMP
dATP can replace ATP with less efficiency
642542
Escherichia virus T5
ADP + dTDP
-
642542
Escherichia virus T5
r
ATP + dTMP
phosphorylated at 70% the rate of dAMP
642542
Escherichia virus T5
ADP + dTDP
-
642542
Escherichia virus T5
r
additional information
very poor acceptor substrates are AMP, CMP or dUMP
642542
Escherichia virus T5
?
-
-
-
-
additional information
no acceptor substrates are dIMP, deoxyxanthylate, 5-hydroxymethyldeoxycytidylate, UMP, GMP, 5-bromo-dUMP, 5-methyl-dCMP or deoxythymidine
642542
Escherichia virus T5
?
-
-
-
-
additional information
no donor substrates are UTP, GTP, CTP, dTTP, dGTP or dCTP
642542
Escherichia virus T5
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Escherichia virus T5
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Escherichia virus T5
7.6
-
substrate dTMP
Escherichia virus T5
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.2
9
about 80% of maximal activity at pH 6.2 and about 65% of maximal activity at pH 9 with substrate dGMP
Escherichia virus T5
6.2
9.4
about 65% of maximal activity at pH 6.2 and about 70% of maximal activity at pH 9.4 with substrate dAMP
Escherichia virus T5
6.3
9
at pH 6.3: about 70% of maximal activity with substrate dTMP, about 85% of maximal activity with substrate dCMP, at pH 9.0: about 50% of maximal activity with substrate dTMP, 65% of maximal activity with substrate dCMP
Escherichia virus T5
Other publictions for EC 2.7.4.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739645
Mikoulinskaia
-
The study of the bacteriophage ...
Escherichia virus T5
Russ. J. Bioorg. Chem.
39
607-618
2013
-
-
1
-
15
-
-
-
-
1
-
4
-
1
-
-
1
-
-
-
5
-
9
1
1
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
15
-
-
-
-
-
-
1
-
4
-
-
-
1
-
-
5
-
9
1
1
-
-
-
2
-
-
-
-
1
1
-
-
-
661371
Bao
Cloning of deoxynucleoside mon ...
Saccharomyces cerevisiae
Biotechnol. Bioeng.
93
572-580
2006
-
-
1
-
-
-
-
8
-
1
4
1
-
4
-
-
-
-
-
-
4
-
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
8
-
1
4
1
-
-
-
-
-
-
4
-
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
663294
Mikoulinskaia
Identification, cloning, and e ...
Escherichia virus T5
Protein Expr. Purif.
33
166-175
2004
-
-
1
-
-
-
-
-
-
-
2
1
-
3
-
-
1
-
-
-
8
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
1
-
-
8
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642544
Mikoulinskaia
Purification and characterizat ...
Escherichia virus T5, no activity in Escherichia coli
Protein Expr. Purif.
27
195-201
2003
-
-
-
-
-
-
-
-
-
-
2
1
-
4
-
-
1
-
-
-
2
1
5
1
-
-
4
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
1
-
-
2
1
5
1
-
-
4
-
1
1
-
1
-
-
-
-
-
-
209854
Allen
T4 phage deoxyribonucleotide-s ...
Escherichia virus T4, no activity in Escherichia coli
J. Biol. Chem.
258
5746-5753
1983
-
-
-
-
-
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642542
Bessman
The enzymology of virus-infect ...
Escherichia virus T5, no activity in Escherichia coli
J. Biol. Chem.
240
439-445
1965
-
-
-
-
-
-
3
6
-
5
-
1
-
2
-
-
1
-
-
-
1
2
12
-
1
-
-
-
2
3
-
-
3
-
-
-
-
-
-
-
-
-
-
3
3
6
-
5
-
1
-
-
-
1
-
-
1
2
12
-
1
-
-
-
2
3
-
-
-
-
-
-
-
-