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Literature summary for 2.7.4.10 extracted from
- Expression, purification, crystallization and preliminary X-ray analysis of Plasmodium falciparum GTP:AMP phosphotransferase (2012), Acta Crystallogr. Sect. F, 68, 671-674.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 (DE3) Rosetta 2 cells | Plasmodium falciparum |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting drop vapor diffusion method, using 1.9-2.1 M ammonium sulfate, 0.1 M Tris-HCl pH 8.0 | Plasmodium falciparum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Plasmodium falciparum | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
x * 30000, SDS-PAGE | Plasmodium falciparum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium falciparum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisTrap column chromatography and Superdex 200 gel filtration | Plasmodium falciparum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + AMP | - |
Plasmodium falciparum | GDP + ADP | - |
r |  |
| additional information | the enzyme does not accept ATP as a substrate | Plasmodium falciparum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30000, SDS-PAGE | Plasmodium falciparum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GAK | - |
Plasmodium falciparum |
| GTP:AMP phosphotransferase | - |
Plasmodium falciparum |
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