General Stability | Organism |
---|---|
ammonium sulfate or substrates enhance stability | Bos taurus |
freeze-thawing, dilutions, or low ionic strength decreases activity rapidly | Bos taurus |
stability increases during purification | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | reverse reaction, kinetics | Bos taurus | |
AMP | free form, Mg2+ reverses | Bos taurus | |
ATP | - |
Bos taurus | |
GDP | reverse reaction, kinetics | Bos taurus | |
GTP | kinetics | Bos taurus | |
Mg2+ | weak, above 4 mM, activates below | Bos taurus | |
additional information | no inhibition by EDTA, GSH, cysteamine or high phosphate concentrations | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
GDP | pH 8.0, with ADP | Bos taurus | |
0.033 | - |
AMP | - |
Bos taurus | |
0.056 | - |
GTP | pH 8.0, with AMP | Bos taurus | |
0.29 | - |
ADP | pH 8.0, with GDP | Bos taurus | |
0.63 | - |
ITP | pH 8.0, with AMP | Bos taurus | |
1 | - |
ATP | pH 8.0, with of AMP | Bos taurus | |
7.4 | - |
UTP | pH 8.0, with of AMP | Bos taurus | |
9.1 | - |
CTP | pH 8.0, with of AMP | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | matrix | Bos taurus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation, 50% as effective as Mg2+ | Bos taurus | |
Mg2+ | activation, 4 mM, as good as Mn2+, slightly inhibitory above 4 mM | Bos taurus | |
Mn2+ | activation, as good as Mg2+ | Bos taurus | |
additional information | no activation by EDTA, cysteamine, GSH or high phosphate concentrations | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
gel filtration | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
nucleoside triphosphate + AMP = nucleoside diphosphate + ADP | mechanism | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
135 | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
0°C, partially purified preparation, several weeks | Bos taurus |
room temperature, 1 week | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + AMP | poor substrate, highly specific for AMP, phosphorylation at about 5% the rate of ITP | Bos taurus | 2 ADP | - |
r | |
CTP + AMP | highly specific for AMP, phosphorylation at about 11% the rate of ITP | Bos taurus | CDP + ADP | - |
r | |
GTP + AMP | highly specific for AMP and ADP, in reverse reaction | Bos taurus | GDP + ADP | via nucleotide-enzyme complex, no phosphorylated enzyme intermediate | r | |
GTP + AMP | phosphorylation at about 90% the rate of ITP | Bos taurus | GDP + ADP | via nucleotide-enzyme complex, no phosphorylated enzyme intermediate | r | |
ITP + AMP | best substrate | Bos taurus | IDP + ADP | via nucleotide-enzyme complex, no phosphorylated enzyme intermediate | r | |
ITP + AMP | highly specific for AMP and ADP in reverse reaction | Bos taurus | IDP + ADP | via nucleotide-enzyme complex, no phosphorylated enzyme intermediate | r | |
ITP + AMP | no acceptor substrates are 3'-GMP | Bos taurus | IDP + ADP | via nucleotide-enzyme complex, no phosphorylated enzyme intermediate | r | |
ITP + AMP | not: IMP, CMP, UMP | Bos taurus | IDP + ADP | via nucleotide-enzyme complex, no phosphorylated enzyme intermediate | r | |
UTP + AMP | highly specific for AMP, phosphorylation at about 19% the rate of ITP | Bos taurus | UDP + ADP | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
35% ammonium sulfate, AMP or other substrates enhance thermal stability | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.7 | about half-maximal activity at pH 7 and 9.7 | Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.73 | - |
ATP | pH 8.5, competitive with AMP | Bos taurus | |
0.74 | - |
ATP | pH 8.5, competitive with GTP | Bos taurus | |
0.77 | - |
GDP | pH 8.5 | Bos taurus | |
0.8 | - |
GTP | pH 8.5 | Bos taurus | |
0.9 | - |
ADP | pH 8.5 | Bos taurus | |
1.6 | - |
AMP | pH 8.5 | Bos taurus |