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Literature summary for 2.7.4.1 extracted from
- Formation of polyphosphate by polyphosphate kinases and its relationship to poly(3-hydroxybutyrate) accumulation in Ralstonia eutropha strain H16 (2015), Appl. Environ. Microbiol., 81, 8277-8293.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| ppk1, DNA and amino acid sequence determination and analysis, recombinant expression of eYFP-tagged protein in Escherichia coli DELTAppk strain | Cupriavidus necator |
| ppk1b, DNA and amino acid sequence determination and analysis, recombinant expression of eYFP-tagged protein in Escherichia coli DELTAppk strain | Cupriavidus necator |
| ppk2a, DNA and amino acid sequence determination and analysis, recombinant expression of eYFP-tagged protein in Escherichia coli DELTAppk strain | Cupriavidus necator |
| ppk2b, DNA and amino acid sequence determination and analysis, recombinant expression of eYFP-tagged protein in Escherichia coli DELTAppk strain | Cupriavidus necator |
| ppk2c, DNA and amino acid sequence determination and analysis, recombinant expression of eYFP-tagged protein in Escherichia coli DELTAppk strain | Cupriavidus necator |
| ppk2d, DNA and amino acid sequence determination and analysis, recombinant expression of eYFP-tagged protein in Escherichia coli DELTAppk strain | Cupriavidus necator |
| ppk2e, DNA and amino acid sequence determination and analysis, recombinant expression of eYFP-tagged protein in Escherichia coli DELTAppk strain | Cupriavidus necator |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a chromosomal deletion strain of the enzyme, formation of polyphosphate and poly(3-hydroxybutyrate) granules in Ralstonia eutropha mutants during growth on NB-gluconate medium, overview | Cupriavidus necator |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Cupriavidus necator | 5737 | - |
| additional information | the enzyme is located near one cell pole apart from polyphosphate granules | Cupriavidus necator | - |
- |
| additional information | the isozyme is located in polyphosphate granules | Cupriavidus necator | - |
- |
| soluble | - |
Cupriavidus necator | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30700 | - |
- |
Cupriavidus necator |
| 31100 | - |
- |
Cupriavidus necator |
| 33900 | - |
- |
Cupriavidus necator |
| 36200 | - |
x * 36200, about, sequence calculation | Cupriavidus necator |
| 42100 | - |
- |
Cupriavidus necator |
| 78200 | - |
- |
Cupriavidus necator |
| 79600 | - |
- |
Cupriavidus necator |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (phosphate)n | Cupriavidus necator | - |
ADP + (phosphate)n+1 | - |
? |  |
| ATP + (phosphate)n | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | - |
ADP + (phosphate)n+1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cupriavidus necator | Q0K2G4 | gene ppk2 or ppk1b or H16_B1019 | - |
| Cupriavidus necator | Q0K8Z5 | i.e. Cupriavidus necator, gene ppk1 or H16_A2437 | - |
| Cupriavidus necator | Q0KA91 | gene ppk2e or H16_A1979 | - |
| Cupriavidus necator | Q0KC60 | gene ppk2d or H16_A1271 | - |
| Cupriavidus necator | Q0KCB8 | gene ppk2c or H16_A1212 | - |
| Cupriavidus necator | Q0KCY1 | gene ppk2b or H16_A0997 | - |
| Cupriavidus necator | Q0KF43 | gene ppk2a or H16_A0226 | - |
| Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | Q0K2G4 | gene ppk2 or ppk1b or H16_B1019 | - |
| Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | Q0K8Z5 | i.e. Cupriavidus necator, gene ppk1 or H16_A2437 | - |
| Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | Q0KA91 | gene ppk2e or H16_A1979 | - |
| Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | Q0KC60 | gene ppk2d or H16_A1271 | - |
| Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | Q0KCB8 | gene ppk2c or H16_A1212 | - |
| Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | Q0KCY1 | gene ppk2b or H16_A0997 | - |
| Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | Q0KF43 | gene ppk2a or H16_A0226 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (phosphate)n | - |
Cupriavidus necator | ADP + (phosphate)n+1 | - |
? | |
| ATP + (phosphate)n | - |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | ADP + (phosphate)n+1 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 36200, about, sequence calculation | Cupriavidus necator |
| ? | x * 30700, about, sequence calculation | Cupriavidus necator |
| ? | x * 31100, about, sequence calculation | Cupriavidus necator |
| ? | x * 33900, about, sequence calculation | Cupriavidus necator |
| ? | x * 42100, about, sequence calculation | Cupriavidus necator |
| ? | x * 78200, about, sequence calculation | Cupriavidus necator |
| ? | x * 79600, about, sequence calculation | Cupriavidus necator |
| More | proteome analysis of PPK motif proteins, overview | Cupriavidus necator |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PPK1 | - |
Cupriavidus necator |
| PPK2 | - |
Cupriavidus necator |
| PPK2a | - |
Cupriavidus necator |
| PPK2B | - |
Cupriavidus necator |
| PPK2c | - |
Cupriavidus necator |
| PPK2d | - |
Cupriavidus necator |
| PPK2e | - |
Cupriavidus necator |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Cupriavidus necator | sequence calculation | - |
5.8 |
| Cupriavidus necator | sequence calculation | - |
5.9 |
| Cupriavidus necator | sequence calculation | - |
6.7 |
| Cupriavidus necator | sequence calculation | - |
7.8 |
| Cupriavidus necator | sequence calculation | - |
9.1 |
| Cupriavidus necator | sequence calculation | - |
9.4 |
| Cupriavidus necator | sequence calculation | - |
9.6 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | identification of a protein PhaX as a putative link between poly(3-hydroxybutyrate) and polyphospate metabolism, the A2274 (phaX) gene product is annotated as a hypothetical putative phosphate transport regulator, regulation, overview. Polyphosphate granules in Ralstonia eutropha are often located in the neighborhood of polyhydroxybutyrate granules if both biopolymers are present simultaneously | Cupriavidus necator |
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