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Literature summary for 2.7.4.1 extracted from

  • Jagannathan, V.; Kaur, P.; Datta, S.
    Polyphosphate kinase from M. tuberculosis: an interconnect between the genetic and biochemical role (2010), PLoS ONE, 5, e14336.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL-21 (DE3) cells Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
ADP product inhibition Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 ADP in 50 mM Tris-HCl pH-7.5, 10 mM NH4SO4,10 mM KPO4, pH 7.0, 4 mM MgCl2, 50 mM NaCl, at 37°C Mycobacterium tuberculosis
0.8
-
 ATP in 50 mM Tris-HCl pH-7.5, 10 mM NH4SO4,10 mM KPO4, pH 7.0, 4 mM MgCl2, 50 mM NaCl, at 37°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimum Mg2+ concentration is 5 mM Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
81000
-
 4 * 81000, gel filtration Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Mono-Q column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + (phosphate)n
-
 Mycobacterium tuberculosis ATP + (phosphate)n-1
-
 r
ATP + (phosphate)n
-
 Mycobacterium tuberculosis ADP + (phosphate)n+1
-
 r

Subunits

Subunits Comment Organism
homotetramer 4 * 81000, gel filtration Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
PPK
-
 Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Mycobacterium tuberculosis