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Literature summary for 2.7.4.1 extracted from
- Synthesis of high molecular weight polyphosphate with a partially purified enzyme from Salmonella (1971), J. Gen. Microbiol., 68, 115-122.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | - |
Salmonella enterica subsp. enterica serovar Minnesota |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | 0.25 mM, approx. 50% inhibition, 1 mM, complete inhibition | Salmonella enterica subsp. enterica serovar Minnesota | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.25 | - |
ATP | pH 8.0, 25°C | Salmonella enterica subsp. enterica serovar Minnesota | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | soluble in the presence of nucleic acid, sediments upon ultracentrifugation at 150000 g for 3 h | Salmonella enterica subsp. enterica serovar Minnesota | - |
- |
| spheroplast | - |
Salmonella enterica subsp. enterica serovar Minnesota | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K2SO4 | activation | Salmonella enterica subsp. enterica serovar Minnesota | |
| additional information | not activated by NH4Cl | Salmonella enterica subsp. enterica serovar Minnesota |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Minnesota | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, DEAE-cellulose | Salmonella enterica subsp. enterica serovar Minnesota |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.047 | - |
- |
Salmonella enterica subsp. enterica serovar Minnesota |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (phosphate)n | enzyme synthesizes polyphosphates of 300000-400000 Da | Salmonella enterica subsp. enterica serovar Minnesota | ADP + (phosphate)n+1 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Salmonella enterica subsp. enterica serovar Minnesota |
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Release 2023.1 (January 2023)
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