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Literature summary for 2.7.3.9 extracted from
- Mycoplasma phosphoenolpyruvate-dependent sugar phosphotransferase system: purification and characterization of enzyme I (1977), J. Bacteriol., 131, 988-996.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-ethylmaleimide | irreversible inactivation | Mycoplasma capricolum |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Mycoplasma capricolum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44500 | - |
alpha2,beta,gamma, 2 * 44500 + 1 * 62000 + 1 * 64500, SDS-PAGE, N-terminal amino acid sequence | Mycoplasma capricolum |
| 62000 | - |
alpha2,beta,gamma, 2 * 44500 + 1 * 62000 + 1 * 64500, SDS-PAGE, N-terminal amino acid sequence | Mycoplasma capricolum |
| 64500 | - |
alpha2,beta,gamma, 2 * 44500 + 1 * 62000 + 1 * 64500, SDS-PAGE, N-terminal amino acid sequence | Mycoplasma capricolum |
| 220000 | - |
gel filtration | Mycoplasma capricolum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycoplasma capricolum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mycoplasma capricolum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Mycoplasma capricolum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, phosphate buffer, pH 7.0, protein concentration 1 mg/ml, stable for at least 14 months | Mycoplasma capricolum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + histidine-containing protein | - |
Mycoplasma capricolum | pyruvate + phosphorylated histidine-containing protein | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | alpha2,beta,gamma, 2 * 44500 + 1 * 62000 + 1 * 64500, SDS-PAGE, N-terminal amino acid sequence | Mycoplasma capricolum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mycoplasma capricolum |
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Release 2023.1 (January 2023)
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