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Literature summary for 2.7.3.9 extracted from

  • Saier, M.H.; Schmidt, M.R.; Lin, P.
    Phosphoryl exchange reaction catalyzed by enzyme I of the bacterial phosphoenolpyruvate: sugar phosphotransferase system. Kinetic characterization (1980), J. Biol. Chem., 255, 8579-8584.
    View publication on PubMed
Search for more literature for 2.7.3.9

Activating Compound

Activating Compound Comment Organism Structure
Co2+ activation Salmonella enterica subsp. enterica serovar Typhimurium
Mg2+ activation Salmonella enterica subsp. enterica serovar Typhimurium
Mn2+ activation Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors Comment Organism Structure
2-Bromoacetyl-1-phosphonate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2-phosphoglycerate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
3-phosphoglycerate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
acetylphosphonate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
Bromopyruvate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
Methylacetylphosphonate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
oxalate
-
 Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 phosphoenolpyruvate
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can substitute for Mg2+ Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Reaction

Reaction Comment Organism Reaction ID
phosphoenolpyruvate + protein histidine = pyruvate + protein Npi-phospho-L-histidine in a second reaction step phosphate is transferred to the N-1 position of the imidazole ring of the histidine containing-protein (i.e. HPr), the phospho group of the intermediate phospho-enzyme I can also be transferred to pyruvate or 2-oxobutyrate Salmonella enterica subsp. enterica serovar Typhimurium
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-ketobutyrate + phosphorylated histidine-containing protein
-
 Salmonella enterica subsp. enterica serovar Typhimurium phosphoenolbutyrate + histidine-containing protein
-
 ?
beta-hydroxypyruvate + phosphorylated histidine-containing protein
-
 Salmonella enterica subsp. enterica serovar Typhimurium ? + histidine-containing protein
-
 ?
phosphoenolpyruvate + histidine-containing protein in a second reaction step phosphate is transferred to the N-1 position of the imidazole ring of the histidine containing-protein (i.e. HPr), the phospho group of the intermediate phospho-enzyme I can also be transferred to pyruvate or 2-oxobutyrate Salmonella enterica subsp. enterica serovar Typhimurium pyruvate + phosphorylated histidine-containing protein
-
 r
pyruvate + phosphorylated histidine-containing protein
-
 Salmonella enterica subsp. enterica serovar Typhimurium phosphoenolpyruvate + histidine-containing protein
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
47.5
-
 stable up to, phosphoenolpyruvate stabilizes, Mg2+ destabilizes Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
 in presence of Mg2+ Salmonella enterica subsp. enterica serovar Typhimurium