Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activation | Salmonella enterica subsp. enterica serovar Typhimurium | |
Mg2+ | activation | Salmonella enterica subsp. enterica serovar Typhimurium | |
Mn2+ | activation | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Bromoacetyl-1-phosphonate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
2-phosphoglycerate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
3-phosphoglycerate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
acetylphosphonate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
Bromopyruvate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
Methylacetylphosphonate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
oxalate | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
phosphoenolpyruvate | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can substitute for Mg2+ | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
phosphoenolpyruvate + protein histidine = pyruvate + protein Npi-phospho-L-histidine | in a second reaction step phosphate is transferred to the N-1 position of the imidazole ring of the histidine containing-protein (i.e. HPr), the phospho group of the intermediate phospho-enzyme I can also be transferred to pyruvate or 2-oxobutyrate | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-ketobutyrate + phosphorylated histidine-containing protein | - |
Salmonella enterica subsp. enterica serovar Typhimurium | phosphoenolbutyrate + histidine-containing protein | - |
? | |
beta-hydroxypyruvate + phosphorylated histidine-containing protein | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? + histidine-containing protein | - |
? | |
phosphoenolpyruvate + histidine-containing protein | in a second reaction step phosphate is transferred to the N-1 position of the imidazole ring of the histidine containing-protein (i.e. HPr), the phospho group of the intermediate phospho-enzyme I can also be transferred to pyruvate or 2-oxobutyrate | Salmonella enterica subsp. enterica serovar Typhimurium | pyruvate + phosphorylated histidine-containing protein | - |
r | |
pyruvate + phosphorylated histidine-containing protein | - |
Salmonella enterica subsp. enterica serovar Typhimurium | phosphoenolpyruvate + histidine-containing protein | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
47.5 | - |
stable up to, phosphoenolpyruvate stabilizes, Mg2+ destabilizes | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
in presence of Mg2+ | Salmonella enterica subsp. enterica serovar Typhimurium |