General Stability | Organism |
---|---|
freeze-drying of preparations in non-volatile buffers causes almost 40% loss of activity. Freezing and thawing at pH 8.6 in N-ethylmorpholine-hydrochloric acid buffer produces complete inactivation | Megascolides cameroni |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NEM | 0.3 mM, complete inhibition | Megascolides cameroni | |
o-Iodosobenzoate | 0.3 mM, complete inhibition | Megascolides cameroni | |
p-hydroxymercuribenzoate | 0.0001 mM, complete inhibition | Megascolides cameroni | |
Phenyl iodoacetate | 1 mM, complete inhibition | Megascolides cameroni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.4 | - |
phospholombricine | pH 8.6, 30°C | Megascolides cameroni | |
9.1 | - |
D-lombricine | pH 8.6, 30°C | Megascolides cameroni | |
11.7 | - |
L-lombricine | pH 8.6, 30°C | Megascolides cameroni |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM, slight activation in both directions | Megascolides cameroni | |
Co2+ | 5 mM, slight activation in both directions | Megascolides cameroni | |
Mg2+ | 5 mM, activation in both directions | Megascolides cameroni | |
Mn2+ | 5 mM, activation in both directions | Megascolides cameroni | |
additional information | not activated by Ni2+, Cu2+, Fe2+, Fe3+, Al3+, Sn2+, Ba2+, Cd2+, Zn2+, Be2+ | Megascolides cameroni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Megascolides cameroni | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Megascolides cameroni |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Megascolides cameroni | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
31 | - |
D-lombricine | Megascolides cameroni |
49 | - |
L-lombricine | Megascolides cameroni |
Storage Stability | Organism |
---|---|
2°C, pH 7.5, 50% v/v glycerol, several months stable | Megascolides cameroni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-lombricine | - |
Megascolides cameroni | ADP + phospholombricine | - |
r | |
ATP + L-lombricine | - |
Megascolides cameroni | ADP + phospholombricine | - |
r | |
ATP + lombricine | i.e. guanidinoethylphospho-O-serine | Megascolides cameroni | ADP + phospholombricine | - |
r | |
dADP + phospholombricine | at 16% of the activity with ADP | Megascolides cameroni | dATP + lombricine | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
synthesis of ATP | Megascolides cameroni |
8.6 | - |
phosphorylation of lombricine | Megascolides cameroni |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | pH 6.5: about 85% of maximal activity, pH 8.0: about 50% of maximal activity, synthesis of ATP | Megascolides cameroni |
7.5 | 9.5 | pH 7.5: about 65% of maximal activity, pH 9.5: about 45% of maximal activity, phosphorylation of lombricine | Megascolides cameroni |