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Literature summary for 2.7.3.3 extracted from

  • Dong, F.; Zhang, N.; Xie, Z.; Meng, X.; Qian, K.; Ji, C.; Lu, M.; Du, Y.; Wang, J.
    Characterization and in vitro expression of arginine kinase gene in the invasive western flower thrips, Frankliniella occidentalis (2019), Comp. Biochem. Physiol. B, 229, 51-57 .
    View publication on PubMed
Search for more literature for 2.7.3.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Frankliniella occidentalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.79
-
 L-arginine pH 7.5, 30°C Frankliniella occidentalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 SDS-PAGE Frankliniella occidentalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-arginine Frankliniella occidentalis
-
 ADP + Nomega-phospho-L-arginine
-
 ?

Organism

Organism UniProt Comment Textmining
Frankliniella occidentalis A0A3G5HLZ2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Frankliniella occidentalis

Source Tissue

Source Tissue Comment Organism Textmining
larva the enzyme is expressed at all developmental stages. The mRNA level is the highest in the 2-day-old second instar larvae, and the 1-day-old prepupae exhibit the lowest mRNA level Frankliniella occidentalis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-arginine
-
 Frankliniella occidentalis ADP + Nomega-phospho-L-arginine
-
 ?
ATP + L-arginine the enzyme is highly stereo specific for L-arginine versus D-arginine Frankliniella occidentalis ADP + Nomega-phospho-L-arginine
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Frankliniella occidentalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.74
-
 L-arginine pH 7.5, 30°C Frankliniella occidentalis

pI Value

Organism Comment pI Value Maximum pI Value
Frankliniella occidentalis calculated from sequence
-
 5.64

Expression

Organism Comment Expression
Frankliniella occidentalis mRNA expression of FoAK is developmentally regulated. The mRNA level is the highest in the 2-day-old second instar larvae, and the 1-day-old prepupae exhibit the lowest mRNA level down
Frankliniella occidentalis mRNA expression of FoAK is developmentally regulated. The mRNA level is the highest in the 2-day-old second instar larvae, and the 1-day-old prepupae exhibit the lowest mRNA level up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.76
-
 L-arginine pH 7.5, 30°C Frankliniella occidentalis